Steinernema aciari sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Guangdong, China

• Published in: Journal of invertebrate pathology 2005, Vol.88 (1), p.58-69
• Main Authors: Qiu, L., Yan, X., Zhou, Y., Nguyen, K.B., Pang, Y.
• Format: Article
• Language: English
• Subjects: 28S rDNA sequence; animal morphology; animal taxonomy; Animals; Base Sequence; Biological and medical sciences; Biological control; body measurements; China; Control; crossing; DNA, Helminth; Entomopathogenic nematode; entomopathogenic nematodes; Female; Fundamental and applied biological sciences. Psychology; Identification; internal transcribed spacers; Invertebrates; Male; Microscopy, Electron, Scanning; Molecular Sequence Data; molecular systematics; morphometry; Nemathelminthia. Plathelmintha; Nematoda; new species; nucleotide sequences; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Polymerase Chain Reaction; Protozoa. Invertebrates; rDNA ITS sequence; Rhabditida - anatomy & histology; Rhabditida - classification; Rhabditida - physiology; ribosomal DNA; Soil Microbiology; Steinernema; Steinernema aciari sp. n; Steinernematidae; Systematics. Geographical distribution; Taxonomy
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2004.09.004

A new species of entomopathogenic nematode, Steinernema aciari sp. n. was described. It was recovered from a soil sample collected from Haimen town, Shantou district in the eastern coast of Guangdong province, the People’s Republic of China during a survey for entomopathogenic nematodes. S. aciari sp. n. belongs to the Steinernema glaseri group. It can be separated from all described Steinernema species by the combined morphological and morphometrical characters of various stages of the nematodes. For male, the new species can be recognized by spicule length (86 ± 6.3 μm); spicule tip blunt with a hook-like structure; gubernaculum with a short and Y-shaped cuneus and corpus well-separated posteriorly. For infective juvenile, the combination of the following characters: body length (1113 ± 68 μm), distance from anterior end to excretory pore (95 ± 3.7 μm), tail length (78 ± 5.2 μm), and E % (123 ± 7) can be used to differentiate the new species from other nematodes. For female, the tail (conoid with a long mamillate terminus and a distinct postanal swelling) and vulva (slightly protruding from body surface with conspicuous double flapped epiptygma) shapes can be used as diagnostic characters for the new species. The new species can also be distinguished from other Steinernema species by DNA sequences of either a partial 28S rDNA or the internal transcribed spacer regions of rDNA, and from the close related species S. glaseri, Steinernema longicaudum CWL05, and Steinernema guangdongense by cross-breeding test.