Unsaturated diether lipids in the psychrotrophic archaeon Halorubrum lacusprofundi

• Published in: Systematic and applied microbiology 2005-01, Vol.28 (1), p.19-26
• Main Authors: Gibson, John A.E., Miller, Matthew R., Davies, Noel W., Neill, Glen P., Nichols, David S., Volkman, John K.
• Format: Article
• Language: English
• Subjects: Antarctica; APCI; Archaea; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biogenesis of cell structures, supramolecular organization; Biological and medical sciences; Electrospray; Fatty Acids, Unsaturated - isolation & purification; Fundamental and applied biological sciences. Psychology; Glycolipids - analysis; Glycolipids - isolation & purification; Halobacteriaceae - chemistry; Halobacteriaceae - growth & development; Halophile; Mass Spectrometry; Membrane Lipids - analysis; Membrane Lipids - isolation & purification; Microbiology; Phospholipids - analysis; Phospholipids - isolation & purification; Psychrotroph; Temperature; Temperature-dependence; Unsaturated ether lipids
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2004.09.004

The major phospholipids of Halorubrum lacusprofundi grown at 25 °C were archaeol phosphatidylglycerol, archaeol phosphatidylglycerylsulphate and archaeol phosphatidylglycerylphosphate methyl ester. Glycolipids included a monoglycosyl archaeol and the sulphate ester of a diglycosyl archaeol. Cultures grown at 12 °C contained the same suite of phospho- and glycolipids, with the addition of a series of unsaturated analogues with up to six double bonds. The patterns of unsaturation were similar for all the phospholipid series, but a different pattern occurred in the glycolipids. The analytical techniques used in this study allow facile detection of unsaturated archaeal cell membrane lipids that are degraded by commonly used chemical derivatization procedures.