Structure of the O-polysaccharide and serological studies of the lipopolysaccharide of Proteus penneri 60 classified into a new Proteus serogroup O70

An alkali-treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1H, 13C and 31P NMR spectroscopy, and the following structure of the linear pentasaccharide–phosphate repeating unit of the O-polysaccharide was established: 6 ) - α - d - Gal p - ( 1 → 3 ) - α - l...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 2005-03, Vol.43 (3), p.351-356
Main Authors: Zych, Krystyna, Perepelov, Andrei, Baranowska, Agata, Zabłotni, Agnieszka, Shashkov, Alexander S., Knirel, Yuriy A., Sidorczyk, Zygmunt
Format: Article
Language:English
Subjects:
4-Acetamido-4,6-dideoxy- d-glucose
Animals
Antibodies, Bacterial
Bacterial polysaccharide structure
Carbohydrate Sequence
Cross Reactions
Cross-reactivity
Epitopes - chemistry
Homology
Humans
Lipopolysaccharide
Lipopolysaccharides
Lipopolysaccharides - chemistry
Lipopolysaccharides - immunology
Magnetic resonance spectroscopy
Molecular Sequence Data
Molecular Structure
NMR
NMR spectroscopy
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular
O Antigens - chemistry
O Antigens - immunology
O-antigen
O-serogroup
Organic chemistry
Polysaccharides
Proteus penneri
Proteus penneri - chemistry
Proteus penneri - classification
Proteus penneri - immunology
Proteus penneri - pathogenicity
Proteus vulgaris
Rabbits
Saccharides
Serotyping
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Summary:An alkali-treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1H, 13C and 31P NMR spectroscopy, and the following structure of the linear pentasaccharide–phosphate repeating unit of the O-polysaccharide was established: 6 ) - α - d - Gal p - ( 1 → 3 ) - α - l - Fuc p NAc - ( 1 → 3 ) - α - d - Glc p NAc - ( 1 → 3 ) - β - d - Qui p 4 NAc - ( 1 → 6 ) - α - d - Glcp - 1 - P - ( O → Rabbit polyclonal O-antiserum against P. penneri 60 reacted with both core and O-polysaccharide moieties of the homologous LPS. Based on the unique O-polysaccharide structure and serological data, we propose to classify P. penneri 60 into a new, separate Proteus serogroup O70. A weak cross-reactivity of P. penneri 60 O-antiserum with the lipopolysaccharide of Proteus vulgaris O8, O15 and O19 was observed and discussed in view of the chemical structures of the O-polysaccharides.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1016/j.femsim.2004.09.004