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Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR
Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non-pathogenic species of Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10...
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Published in: | FEMS immunology and medical microbiology 2005-03, Vol.43 (3), p.413-417 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of
Burkholderia mallei that causes glanders and non-pathogenic species of
Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10-bp repetitive element in
B. pseudomallei and
B. mallei amplifying PCR fragment of varying sizes between 400–700 bp, a unique sequence in
B. thailandensis amplifying a PCR fragment of 308 bp and the metalloprotease gene amplifying a PCR fragment of 245 bp in
B. pseudomallei and
B. thailandensis. The multiplex PCR not only can differentiate the three
Burkholderia species but can also be used for epidemiological typing of
B. pseudomallei and
B. mallei strains. |
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ISSN: | 0928-8244 1574-695X 2049-632X |
DOI: | 10.1016/j.femsim.2004.10.008 |