C5a-induced in vitro basophil activation in patients with chronic urticaria: a pilot study

Summary BACKGROUND: Complement component 5a (C5a) might be involved in the formation of wheals in patients with chronic urticaria (CU). We sought to compare the in vitro responsiveness of basophils to C5a in patients with CU and in a control group. METHODS: Basophil surface expression of activation...

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Published in:Wiener Klinische Wochenschrift 2009, Vol.121 (9-10), p.339-343
Main Authors: Korosec, Peter, Subic, Tjasa, Adamic, Katja, Silar, Mira, Kosnik, Mitja
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, CD - immunology
Basophil Degranulation Test
Basophils - immunology
Chronic Disease
Complement C5a - immunology
Endocrinology
Female
Flow Cytometry
Gastroenterology
Humans
Immunoglobulin E - immunology
In Vitro Techniques
Internal Medicine
Male
Medicine
Medicine & Public Health
Middle Aged
Original Article
Pilot Projects
Platelet Membrane Glycoproteins - immunology
Pneumology/Respiratory System
Receptors, IgE - immunology
Tetraspanin 30
Urticaria - immunology
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Summary:Summary BACKGROUND: Complement component 5a (C5a) might be involved in the formation of wheals in patients with chronic urticaria (CU). We sought to compare the in vitro responsiveness of basophils to C5a in patients with CU and in a control group. METHODS: Basophil surface expression of activation marker CD63 induced by C5a, anti-FcεRI mAb or anti-IgE pAb was measured using flow cytometry in 17 patients with CU and in 10 healthy controls. RESULTS: Patients with CU showed significantly greater basophil CD63 surface expression induced by C5a (median [interquartile range]; 16.4% [13–25.1]; P = 0.011) than the group of healthy controls (10.7% [7.2–16.8]). In contrast, basophil CD63 response to anti-IgE and anti-FcεRI was lower in the CU group (12.3% [6–36.3]; 25.9% [12.5–60.5]) than in the control group (51.7% [6.7–84.3]; 62.1% [9.7–89.2]), although not statistically significant. CONCLUSION: Results of this pilot study suggest that patients with CU might have an enhanced basophil response to stimulation with C5a, indicating that further studies in CU basophil responsiveness are needed.
ISSN:0043-5325
1613-7671
DOI:10.1007/s00508-009-1168-9