Eosinophil granulocytes: functional differences of a new isolation kit compared to the isolation with anti-CD16-conjugated MicroBeads

Background/purpose:  Using a new eosinophil isolation kit, we were not able to confirm our previous findings of a delayed apoptosis of eosinophils in atopic dermatitis. Thus, we investigated whether this new isolation kit modulates the functional activity of eosinophils. Methods:  Peripheral blood e...

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Bibliographic Details
Published in:Experimental dermatology 2009-07, Vol.18 (7), p.653-655
Main Authors: Schefzyk, Matthias, Bruder, Manuela, Schmiedl, Andreas, Stephan, Michael, Kapp, Alexander, Wedi, Bettina, Raap, Ulrike
Format: Article
Language:English
Subjects:
Antigens, CD - metabolism
Antigens, Differentiation, T-Lymphocyte - metabolism
Apoptosis
Biological and medical sciences
CD16 negative selection
Cell Separation - methods
Cell Survival
Cells, Cultured
Dermatitis, Atopic - immunology
Dermatitis, Atopic - pathology
Dermatology
eosinophil granulocytes
Eosinophils - cytology
Eosinophils - immunology
fas Receptor - metabolism
Humans
Lectins, C-Type
Medical sciences
MicroBeads
Microspheres
Receptors, IgG
Superoxides - metabolism
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Summary:Background/purpose:  Using a new eosinophil isolation kit, we were not able to confirm our previous findings of a delayed apoptosis of eosinophils in atopic dermatitis. Thus, we investigated whether this new isolation kit modulates the functional activity of eosinophils. Methods:  Peripheral blood eosinophils were isolated with the new isolation kit as well as conventionally with anti‐CD16‐conjugated MicroBeads. We analysed viability, apoptosis, CD69 and CD95 expression, streptavidin binding and superoxide anion release. Results:  Purity of eosinophils was higher using the new isolation kit (P 
ISSN:0906-6705
1600-0625
DOI:10.1111/j.1600-0625.2008.00824.x