Evaluation of a new rapid immunoassay for the detection of Helicobacter pylori in faeces: a prospective pilot study

Summary Background : Detection of Helicobacter pylori antigen in faeces is a valid method to diagnose H. pylori infection. Presently available stool tests are performed in the laboratory, and diagnostic report is delayed. Aim : To evaluate a new rapid stool test in a pre‐treatment setting and to com...

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Bibliographic Details
Published in:Alimentary pharmacology & therapeutics 2005-02, Vol.21 (4), p.485-489
Main Authors: Trevisani, L., Sartori, S., Rossi, M. R., Ruina, M., Matarese, V., Gullini, S., Abbasciano, V.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antigens, Bacterial - analysis
Biological and medical sciences
Digestive system
Epidemiologic Methods
Feces - microbiology
Female
Gastroenterology. Liver. Pancreas. Abdomen
Helicobacter Infections - diagnosis
Helicobacter pylori - immunology
Helicobacter pylori - isolation & purification
Humans
Immunoassay - methods
Immunoenzyme Techniques
Male
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Reagent Kits, Diagnostic
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Summary:Summary Background : Detection of Helicobacter pylori antigen in faeces is a valid method to diagnose H. pylori infection. Presently available stool tests are performed in the laboratory, and diagnostic report is delayed. Aim : To evaluate a new rapid stool test in a pre‐treatment setting and to compare it with a validated laboratory stool test. Methods : A total of 105 patients underwent gastroscopy with brush cytology, and biopsies for histology and rapid urease test, to assess H. pylori presence. Helicobacter pylori‐status was considered positive if at least two tests were positive; negative if all tests were negative; indeterminate if one test was positive and two negative. Stool specimens were tested using either a rapid immunoassay kit (ImmunoCard STAT) or a laboratory enzyme immunoassay kit (Hp StAR). Results : Sixty patients were infected with H. pylori, 44 non‐infected, one indeterminate. The sensitivity and specificity of ImmunoCard STAT were 85 and 93%; those of Hp StAR were 88 and 100% (not significant). Conclusions : ImmunoCard STAT seems a reliable method for detecting H. pylori in untreated patients. It could replace laboratory stool tests, as it is easy and can be performed quickly. These characteristics might be a breakthrough for diagnosing H. pylori in the doctor's office.
ISSN:0269-2813
1365-2036
DOI:10.1111/j.1365-2036.2005.02355.x