lipophilicity indices of flavonoids estimated by reversed-phase liquid chromatography using different computation methods

The chromatographic behavior of some flavonoids was established by RP-HPLC on RP-18 (LiChroCART, LiChrosphere RP-18e), RP-8 (Zorbax, Eclipse XDB-C8), CN (Säulentechnik, LiChrosphere CN100) columns. The mobile phases were mixtures of methanol-water in different volume proportions from 70 to 80% v/v f...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2009-06, Vol.32 (12), p.2066-2074
Main Authors: Briciu, Rodica Domnica, Kot-Wasik, Agata, Namiesnik, Jacek, Sârbu, Costel
Format: Article
Language:English
Subjects:
Algorithms
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Computation methods
Exact sciences and technology
Flavonoids
Flavonoids - analysis
General pharmacology
Humans
Lipophilicity
Medical sciences
Methanol - chemistry
Other chromatographic methods
Pharmacology. Drug treatments
Physicochemical properties. Structure-activity relationships
Principal component analyis
Principal Component Analysis - methods
RP-HPLC
Software
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The chromatographic behavior of some flavonoids was established by RP-HPLC on RP-18 (LiChroCART, LiChrosphere RP-18e), RP-8 (Zorbax, Eclipse XDB-C8), CN (Säulentechnik, LiChrosphere CN100) columns. The mobile phases were mixtures of methanol-water in different volume proportions from 70 to 80% v/v for RP-18 and RP-8, while for the CN column the proportions were between 66 and 70% v/v. The lipophilicity was expressed through different lipophilicity descriptors such as mean of k (mk), mean of log k (mlog k), log kW, S, f₀, and scores of k and log k corresponding to the first principal component. The experimental lipophilicity indices are directly correlated with the computed values, via computer software and internet module, at a high analytical level. Furthermore, the results obtained applying principal component analysis to k and/or log k values allow the prediction and explanation of the interaction involved in the retention mechanism which takes place between the compounds and the employed stationary phases during the development.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900106