The electrochemical behaviour of ferrocene in a photocurable poly(methyl methacrylate-co-2-hydroxylethyl methacrylate) film for a glucose biosensor

A single-step fabrication of a glucose biosensor with simultaneous immobilization of both ferrocene mediator and glucose oxidase in a photocurable methacrylic film consisting of poly(methyl methacrylate-co-2-hydroxylethyl methacrylate) was reported. The entrapped ferrocene showed reversible redox be...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2005-02, Vol.65 (2), p.157-162
Main Authors: Bean, Low Sim, Heng, Lee Yook, Yamin, Bohari Mohd, Ahmad, Musa
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Electrochemistry
Electrodes
Ferrocene
Ferrous Compounds - chemistry
Glucose - analysis
Glucose biosensor
Immobilization
Metallocenes
Methacrylate polymers
Oxidation-Reduction
Photocurable
Polyhydroxyethyl Methacrylate
Polymethyl Methacrylate
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Summary:A single-step fabrication of a glucose biosensor with simultaneous immobilization of both ferrocene mediator and glucose oxidase in a photocurable methacrylic film consisting of poly(methyl methacrylate-co-2-hydroxylethyl methacrylate) was reported. The entrapped ferrocene showed reversible redox behaviour in the photocured film and no significant leaching of both entrapped ferrocene and enzyme glucose oxidase was observed because of the low water absorption properties of the co-polymer films. From electrochemical studies, ferrocene entrapped in the co-polymer film demonstrated slow diffusion properties. A linear glucose response range of 2–11 mM was obtained at low applied potential of +0.25 V. The glucose biosensor fabricated by this photocuring method yielded sensor reproducibility and repeatability with relative standard deviation of
ISSN:1567-5394
DOI:10.1016/j.bioelechem.2004.11.003