Separation and purification of aloe polysaccharides by a combination of membrane ultrafiltration and aqueous two-phase extraction

• Published in: Applied biochemistry and biotechnology 2009-07, Vol.158 (1), p.11-19
• Main Authors: Xing, Jian-min, Li, Fen-fang
• Format: Article
• Language: English
• Subjects: Acrylic Resins - analysis; Acrylic Resins - chemistry; Acrylonitrile; Aloe; Aloe - chemistry; Aloe varavia; Ammonium; ammonium sulfate; Biochemistry; Biological and medical sciences; Biotechnology; Chemical Fractionation - methods; Chemistry; Chemistry and Materials Science; Chromatography; composite polymers; extraction; Fundamental and applied biological sciences. Psychology; glucose; infrared spectroscopy; Membranes; Membranes, Artificial; nonfood plant products; optimization; Polymers; polysaccharides; Polysaccharides - analysis; Polysaccharides - isolation & purification; Polystyrenes - chemistry; pulp; Saccharides; scanning electron microscopy; separation; Sodium chloride; Spectroscopy, Fourier Transform Infrared; Styrene; Thermogravimetry; Ultrafiltration; Water - chemistry
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-009-8641-9

A two-step process was developed for the purification of polysaccharides from the pulp of Aloe varavia using aqueous two-phase system (ATPS) extraction and a novel copolymer ultrafiltration membrane. The first step was ATPS under optimal separations conditions using a total composition of 18% PEG2000, 25% ammonium sulfate, pH 3.0, and 0.3 M NaCl. To form the copolymer membrane, poly(acrylonitrile-acrylamide-styrene) was prepared by solution polycondensation using azoisobutyronitrile as initiator. Then, membranes were formed from the dissolved copolymer by the phase inversion method. Copolymer structure was investigated by infrared spectrum and thermogravimetric analysis (TGA). The copolymer membrane surface and cross section were observed by scanning electron microscopy. The water flux of this membrane was 26.33 mL/(cm² h), and retention was 96% for bovine serum albumin and 34% for dextran T40000. The separation and purification of aloe polysaccharide were carried using this copolymer membrane following ATPS. The TGA of aloe polysaccharide demonstrated a high purity of the polysaccharide. By gas chromatographic analysis, it was shown that mannose is the main monosaccharide in the aloe polysaccharide, and only a few glucose residues are present.