Involvement of polyamines in evening primrose extract-induced apoptosis in Ehrlich ascites tumor cells

We previously demonstrated that evening primrose extract (EPE) induced apoptosis and inhibited the DNA synthesis in Ehrlich ascites tumor cells (EATC) and suggested that EPE-induced inhibition of the growth of EATC are via at least two pathway differentially modulated by reactive oxygen species, not...

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Bibliographic Details
Published in:Amino acids 2005-02, Vol.28 (1), p.21-27
Main Authors: Arimura, T, Kojima-Yuasa, A, Tatsumi, Y, Kennedy, D O, Matsui-Yuasa, I
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Carcinoma, Ehrlich Tumor - drug therapy
Carcinoma, Ehrlich Tumor - pathology
Cell Survival - drug effects
Cells
Cellular biology
Chromatography, High Pressure Liquid - methods
Deoxyribonucleic acid
DNA, Neoplasm - biosynthesis
DNA, Neoplasm - drug effects
Dose-Response Relationship, Drug
Evening
Hydrogen peroxide
Oenothera biennis - chemistry
Pathways
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polyamines
Polyamines - analysis
Polyamines - chemistry
Polyamines - pharmacology
Putrescine - pharmacology
Spermidine - pharmacology
Spermine - pharmacology
Synthesis
Tumor Cells, Cultured
Tumors
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Summary:We previously demonstrated that evening primrose extract (EPE) induced apoptosis and inhibited the DNA synthesis in Ehrlich ascites tumor cells (EATC) and suggested that EPE-induced inhibition of the growth of EATC are via at least two pathway differentially modulated by reactive oxygen species, notably intracellular peroxides. These are (a) the EPE-induced apoptosis pathway which is dependent on increases in hydrogen peroxide and (b) the EPE-induced inhibition of cell proliferation which is hydrogen peroxide independent. In this study, EPE brought about a significant decrease in intracellular polyamine levels. Furthermore, the addition of polyamines reversed the EPE-induced decrease in cell viability and suppressed the EPE-induced increase in intracellular hydrogen peroxides. However, the addition of polyamines did not reverse EPE-induced decrease in DNA synthesis and phosphorylation of Rb protein, and EPE-induced translocation of AIF. These results suggest the involvement of polyamines in the EPE-induced apoptosis pathway which is dependent on increase in hydrogen peroxide.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-004-0155-8