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Cloning and use of sC as homologous marker for Aspergillus niger transformation
The sC sequence from Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the sC gene amplified by PCR from the A. niger genome was provided with Aspergillus nidulans expression signals ( gpdA promoter and trpC terminator). This...
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| Published in: | Journal of microbiological methods 2005-05, Vol.61 (2), p.219-224 |
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| Language: | English |
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| container_end_page | 224 |
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| container_title | Journal of microbiological methods |
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| creator | Varadarajalu, Lakshmi P. Punekar, Narayan S. |
| description | The
sC sequence from
Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the
sC gene amplified by PCR from the
A. niger genome was provided with
Aspergillus nidulans expression signals (
gpdA promoter and
trpC terminator). This chimeric construct was used to successfully transform a spontaneous
sC
− isolate of
A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type
A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the
sC
niger
construct, driven by
PgpdA, as a marker for
A. niger transformation. |
| doi_str_mv | 10.1016/j.mimet.2004.11.022 |
| format | article |
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sC sequence from
Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the
sC gene amplified by PCR from the
A. niger genome was provided with
Aspergillus nidulans expression signals (
gpdA promoter and
trpC terminator). This chimeric construct was used to successfully transform a spontaneous
sC
− isolate of
A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type
A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the
sC
niger
construct, driven by
PgpdA, as a marker for
A. niger transformation.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2004.11.022</identifier><identifier>PMID: 15722148</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Aspergillus nidulans ; Aspergillus nidulans - genetics ; Aspergillus niger ; Aspergillus niger - enzymology ; Aspergillus niger - genetics ; ATP sulfurylase activity ; Biological and medical sciences ; Blotting, Southern ; Cloning, Molecular ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic Markers - genetics ; Genetic Markers - physiology ; Marker ; Microbiology ; Miscellaneous ; Mycology ; Polymerase Chain Reaction ; sC gene ; Sulfate Adenylyltransferase - genetics ; Sulfate Adenylyltransferase - metabolism ; Transformation ; Transformation, Genetic - physiology</subject><ispartof>Journal of microbiological methods, 2005-05, Vol.61 (2), p.219-224</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-2f259972e1cdaa4364ed026a950951135beb9c437d7eee246d1ff68a5d7c4ddd3</citedby><cites>FETCH-LOGICAL-c449t-2f259972e1cdaa4364ed026a950951135beb9c437d7eee246d1ff68a5d7c4ddd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16558913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15722148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Varadarajalu, Lakshmi P.</creatorcontrib><creatorcontrib>Punekar, Narayan S.</creatorcontrib><title>Cloning and use of sC as homologous marker for Aspergillus niger transformation</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The
sC sequence from
Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the
sC gene amplified by PCR from the
A. niger genome was provided with
Aspergillus nidulans expression signals (
gpdA promoter and
trpC terminator). This chimeric construct was used to successfully transform a spontaneous
sC
− isolate of
A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type
A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the
sC
niger
construct, driven by
PgpdA, as a marker for
A. niger transformation.</description><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - genetics</subject><subject>ATP sulfurylase activity</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers - genetics</subject><subject>Genetic Markers - physiology</subject><subject>Marker</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Polymerase Chain Reaction</subject><subject>sC gene</subject><subject>Sulfate Adenylyltransferase - genetics</subject><subject>Sulfate Adenylyltransferase - metabolism</subject><subject>Transformation</subject><subject>Transformation, Genetic - physiology</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhoMo7rj6CwTJRW_dpvLRSQ4elsEvWNiLnkMmqR4zdnfGpEfw35t1BvbmngqqniqK9yHkNbAeGAzvD_2cZlx7zpjsAXrG-ROyAaN5Z4SyT8mmUbrTDPgVeVHrgTFQQprn5AqU5hyk2ZC77ZSXtOypXyI9VaR5pHVLfaU_8pynvM-nSmdffmKhYy70ph6x7NM0tfaS9q27Fr_UNpr9mvLykjwb_VTx1aVek--fPn7bfulu7z5_3d7cdkFKu3Z85MpazRFC9F6KQWJkfPBWMasAhNrhzgYpdNSIyOUQYRwH41XUQcYYxTV5d757LPnXCevq5lQDTpNfsL3sBi2lAM4eBcEOUhojHwe1AiYNb6A4g6HkWguO7lhSi-iPA-buzbiD-2fG3ZtxAK6ZaVtvLudPuxnjw85FRQPeXgBfg5_GFmtI9YEblDIWROM-nDls8f5OWFwNCZeAMRUMq4s5_feRv8p6rQk</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Varadarajalu, Lakshmi P.</creator><creator>Punekar, Narayan S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Cloning and use of sC as homologous marker for Aspergillus niger transformation</title><author>Varadarajalu, Lakshmi P. ; Punekar, Narayan S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-2f259972e1cdaa4364ed026a950951135beb9c437d7eee246d1ff68a5d7c4ddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - genetics</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - enzymology</topic><topic>Aspergillus niger - genetics</topic><topic>ATP sulfurylase activity</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers - genetics</topic><topic>Genetic Markers - physiology</topic><topic>Marker</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Polymerase Chain Reaction</topic><topic>sC gene</topic><topic>Sulfate Adenylyltransferase - genetics</topic><topic>Sulfate Adenylyltransferase - metabolism</topic><topic>Transformation</topic><topic>Transformation, Genetic - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Varadarajalu, Lakshmi P.</creatorcontrib><creatorcontrib>Punekar, Narayan S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Varadarajalu, Lakshmi P.</au><au>Punekar, Narayan S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and use of sC as homologous marker for Aspergillus niger transformation</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>61</volume><issue>2</issue><spage>219</spage><epage>224</epage><pages>219-224</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>The
sC sequence from
Aspergillus niger was cloned and developed into a homologous marker system for genetic transformation. The coding region of the
sC gene amplified by PCR from the
A. niger genome was provided with
Aspergillus nidulans expression signals (
gpdA promoter and
trpC terminator). This chimeric construct was used to successfully transform a spontaneous
sC
− isolate of
A. niger to prototrophy. The transformants analyzed by Southern analysis showed integration of multiple copies of the transforming DNA. They also exhibited much higher ATP sulfurylase activity than the wild-type
A. niger strain reinforcing the molecular data. This demonstrates the usefulness of the
sC
niger
construct, driven by
PgpdA, as a marker for
A. niger transformation.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>15722148</pmid><doi>10.1016/j.mimet.2004.11.022</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2005-05, Vol.61 (2), p.219-224 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67443120 |
| source | ScienceDirect Journals |
| subjects | Aspergillus nidulans Aspergillus nidulans - genetics Aspergillus niger Aspergillus niger - enzymology Aspergillus niger - genetics ATP sulfurylase activity Biological and medical sciences Blotting, Southern Cloning, Molecular DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Genetic Markers - genetics Genetic Markers - physiology Marker Microbiology Miscellaneous Mycology Polymerase Chain Reaction sC gene Sulfate Adenylyltransferase - genetics Sulfate Adenylyltransferase - metabolism Transformation Transformation, Genetic - physiology |
| title | Cloning and use of sC as homologous marker for Aspergillus niger transformation |
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