Influence of nanoporous alumina membranes on long-term osteoblast response

A major goal of bone tissue engineering is to design better scaffold configuration and materials to better control osteoblast behavior. Nanoporous architecture has been shown to significantly affect cellular response. In this work, nanoporous alumina membranes were fabricated by a two-step anodizati...

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Bibliographic Details
Published in:Biomaterials 2005-08, Vol.26 (22), p.4516-4522
Main Authors: Popat, Ketul C., Leary Swan, Erin E., Mukhatyar, Vivek, Chatvanichkul, Kwan-Isara, Mor, Gopal K., Grimes, Craig A., Desai, Tejal A.
Format: Article
Language:English
Subjects:
Aluminum - chemistry
Bone tissue engineering
Cell Adhesion
Cell Line
Cell Proliferation
Extracellular Matrix
Humans
Matrix production
Nanoporous alumina
Nanotechnology
Osteoblast adhesion
Osteoblasts - cytology
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Summary:A major goal of bone tissue engineering is to design better scaffold configuration and materials to better control osteoblast behavior. Nanoporous architecture has been shown to significantly affect cellular response. In this work, nanoporous alumina membranes were fabricated by a two-step anodization method to investigate bone cell response. Osteoblasts were seeded on nanoporous alumina membranes to investigate both short-term adhesion and proliferation and long-term functionality and matrix production. Cell adhesion and proliferation were characterized using a standard MTT assay and cell counting. The total protein content was measured after cell lysis using the BCA assay. Matrix production was characterized in terms of surface concentrations of calcium and phosphorous, components of bone matrix, using X-ray photoelectron spectroscopy (XPS). The results from nanoporous alumina membranes were compared with those of amorphous alumina, aluminum, commercially available ANOPORE TM and glass. Results indicate improved osteoblast adhesion and proliferation and increased matrix production after 4 weeks of study.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2004.11.026