Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen

• Published in: Animal reproduction science 2005-03, Vol.86 (1), p.163-173
• Main Authors: Cassani, Paula, Beconi, Martha T., O’Flaherty, Cristian
• Format: Article
• Language: English
• Subjects: Animals; Dog; dogs; Dogs - physiology; enzyme activity; Lipid Peroxidation; Male; males; Microscopy, Interference - veterinary; phospholipids; plasma membrane; Reactive oxygen species; semen; Semen - cytology; Semen - enzymology; Sperm Count - veterinary; Sperm motility; Sperm Motility - physiology; Spermatozoa; Spermatozoa - abnormalities; Spermatozoa - cytology; spermiogenesis; Superoxide dismutase; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; viability
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2004.06.006

There is a lack of information regarding biochemical studies on canine semen. The knowledge of canine sperm metabolism is important because this species has differences in fertility among males, but biochemical studies are not well developed in this species. Reactive oxygen species (ROS) are active molecules produced during the oxygen reduction that have been implicated in several deleterious effects on the function and viability of spermatozoa when they are produced at high concentrations. Peroxidation of the plasma membrane phospholipids leads to a loss of motility, viability, and cytosolic elements, metabolic changes and structural alterations in spermatozoa. The most important antioxidant enzyme involved in ROS detoxification in mammalian spermatozoa is superoxide dismutase (SOD). The aims of the present study were to determine the presence of SOD-like activity in different fractions of canine semen, and to determine the relationship between SOD-like activity and different morphological, dynamical and biochemical parameters in canine spermatozoa. We demonstrated the presence of SOD-like activity in canine seminal plasma and spermatozoa; the negative correlation between SOD-like activity and lipid peroxidation concentrations in spermatozoa suggests a protective effect of this enzymatic activity against the potential oxidative stress in canine spermatozoa. A significant negative correlation between percentage of abnormal spermatozoa, and the percentage of progressive motility or vigor was found. Abnormal spermatozoa not only showed a deformed shape, moreover there was an energetic motility system failure. The presence of SOD-like activity in seminal plasma, spermatic, and postspermatic fractions of canine semen was demonstrated for the first time.