Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast

We describe a full-field phase-gradient imaging method: asymmetric illumination-based differential phase contrast (AIDPC). Imaging properties of AIDPC are evaluated using the phase-gradient transfer-function approach and elucidated with experimental images of an optical fiber and a histochemical pre...

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Bibliographic Details
Published in:Optics letters 2009-07, Vol.34 (13), p.1924-1926
Main Authors: MEHTA, Shalin B, SHEPPARD, Colin J. R
Format: Article
Language:English
Subjects:
Absorption
Conventional optical microscopes
Exact sciences and technology
Fundamental areas of phenomenology (including applications)
Imaging and optical processing
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Lighting - instrumentation
Lighting - methods
Microscopy, Phase-Contrast - instrumentation
Microscopy, Phase-Contrast - methods
Modulation and optical transfer functions
Muscle, Skeletal - metabolism
Optical Fibers
Optical instruments, equipment and techniques
Optics
Phase retrieval
Physics
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Description
Summary:We describe a full-field phase-gradient imaging method: asymmetric illumination-based differential phase contrast (AIDPC). Imaging properties of AIDPC are evaluated using the phase-gradient transfer-function approach and elucidated with experimental images of an optical fiber and a histochemical preparation of a skeletal muscle section. In comparison with full-field differential interference contrast, AIDPC does not require phase shifting for quantitative imaging of phase gradient, provides artifact-free images of birefringent specimens, requires shorter camera exposure, and has larger depth of focus. It is amenable to transfer-function engineering, simultaneous fluorescence imaging, and automated live cell imaging.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.34.001924