Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis

• Published in: The New phytologist 2005-03, Vol.165 (3), p.801-806
• Main Authors: Moore, B. J., Donnison, I. S., Harper, J. A., Armstead, I. P., King, J., Thomas, H., Jones, R. N., Jones, T. H., Thomas, H. M., Morgan, W. G., Thomas, A., Ougham, H. J., Huang, L., Fentem, T., Roberts, L. A., King, I. P.
• Format: Article
• Language: English
• Subjects: amplified fragment length polymorphism; BAC libraries; Biological and medical sciences; chlorophyll breakdown; Chromosome Mapping; Chromosome polymorphism; Chromosomes; Chromosomes, Plant; cloning; Crosses, Genetic; Diploidy; Festuca - genetics; Festuca - physiology; Festuca pratensis; Fundamental and applied biological sciences. Psychology; Genes; Genes, Plant; Genetic Linkage; Genetic mapping; Genetic mutation; Genetic screening; Genotypes; Hybridity; interspecific hybridization; introgression; introgression mapping; leaf development; Lolium - genetics; Lolium multiflorum; Lolium/Festuca pratensis interspecific hybrids; mapping; mutants; Mutation; Nucleic Acid Amplification Techniques; Phenotype; Plant physiology and development; plant proteins; Plants; Polymorphism, Genetic; senescence; Senescence and abscission; Vegetative apparatus, growth and morphogenesis. Senescence
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.2004.01269.x

• Intergeneric hybrids between Lolium multiflorum and Festuca pratensis (Lm/Fp) and their derivatives exhibit a unique combination of genetic and cytogenetic characteristics: chromosomes undergo a high frequency of homoeologous recombination at meiosis; the chromosomes of the two species can easily be discriminated by genomic in situ hybridization (GISH); recombination occurs along the entire length of homoeologous bivalents; a high frequency of marker polymorphism is observed between the two species. • This combination of characters has been used to transfer and isolate a F. pratensis chromosome segment carrying a mutant 'stay-green' gene conferring a disrupted leaf senescence phenotype into L. multiflorum. • The genetic location within the introgressed F. pratensis segment of the senescence gene has been mapped using amplified fragment length polymorphisms (AFLPs), and F. pratensis-specific AFLP markers closely flanking the green gene have been cloned. • The use of these cloned sequences as markers for the stay-green locus in marker-assisted selection programmes has been tested. The potential application of Lm/Fp introgressions as a tool for the map-based cloning of introgressed Fp genes is discussed.