Fluorescence Detection of Single-Nucleotide Polymorphisms with a Single, Self-Complementary, Triple-Stem DNA Probe

Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational cha...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2009-06, Vol.48 (24), p.4354-4358
Main Authors: Xiao, Yi, Plakos, Kory J.I, Lou, Xinhui, White, Ryan J, Qian, Jiangrong, Plaxco, Kevin W, Soh, H. Tom
Format: Article
Language:English
Subjects:
Base Sequence
DNA
DNA Probes - chemistry
DNA Probes - genetics
Fluorescent Dyes - chemistry
fluorescent probes
Polymorphism, Single Nucleotide
self‐complementary sequences
single‐nucleotide polymorphisms
Spectrometry, Fluorescence
Thermodynamics
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Summary:Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F=fluorophore, Q=quencher).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200900369