Pine microsatellite markers allow roots and ectomycorrhizas to be linked to individual trees

• Linking roots and ectomycorrhizas (EcM) to individual host trees in the field is required to test whether individual trees support different ectomycorrhizal communities. • Here we describe a method that identifies the source of EcM roots by PCR of polymorphic pine nuclear microsatellite loci using...

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Bibliographic Details
Published in:The New phytologist 2005, Vol.165 (1), p.295-304
Main Authors: Saari, S. K., Campbell, C. D., Russell, J., Alexander, I. J., Anderson, I. C.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
DNA Fingerprinting
Economic plant physiology
ectomycorrhizae
Ectomycorrhizas
forest trees
Fundamental and applied biological sciences. Psychology
Fungi
genetic variation
Genotypes
host genotype
internal transcribed spacers
microsatellite fingerprinting
Microsatellite Repeats
Microsatellites
Mycorrhizae - genetics
mycorrhizal fungi
Parasitism and symbiosis
Pine trees
Pinus sylvestris
Pinus sylvestris - genetics
Pinus sylvestris - microbiology
Pinus sylvestris var. scotica
plant genetics
Plant physiology and development
Plant roots
Plant Roots - genetics
Plant Roots - microbiology
Plants
Polymerase Chain Reaction
root ecology
Root tips
Scots pine (Pinus sylvestris)
Symbiosis
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
Trees
woodlands
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Summary:• Linking roots and ectomycorrhizas (EcM) to individual host trees in the field is required to test whether individual trees support different ectomycorrhizal communities. • Here we describe a method that identifies the source of EcM roots by PCR of polymorphic pine nuclear microsatellite loci using fluorescently labelled primers and high-throughput fragment analysis. ITS-PCR can also be performed on the same EcM DNA extract for fungal identification. • The method was tested on five neighbouring Scots pine (Pinus sylvestris var scotica) trees in native woodland. Successful host tree identification from DNA extracts of EcM root tips was achieved for 93% of all root fragments recovered from soil cores. • It was estimated that each individual mature pine sampled was colonised by between 15 and 19 EcM fungi. The most abundant fungal species were found on all five trees, and within the constraints of the sampling scheme, no differences between trees in EcM fungal community structure or composition were detected.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2004.01213.x