Hepatitis C virus genotype distribution in China: Predominance of closely related subtype 1b isolates and existence of new genotype 6 variants

To determine hepatitis C virus (HCV) genotype distribution in China, a total of 148 HCV RNA positive serum samples were collected from nine geographic areas and subjected to RT‐PCR followed by direct DNA sequencing and phylogenetic analysis of the core, E1, and NS5B regions. HCV was genotyped in 139...

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Bibliographic Details
Published in:Journal of medical virology 2005-04, Vol.75 (4), p.538-549
Main Authors: Lu, Ling, Nakano, Tatsunori, He, Yunshao, Fu, Yongshui, Hagedorn, Curt H., Robertson, Betty H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
China
China - epidemiology
DNA sequencing
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genotype
Hepacivirus - classification
Hepacivirus - genetics
Hepatitis C - epidemiology
Hepatitis C - virology
hepatitis C virus
Human viral diseases
Humans
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Molecular Sequence Data
phylogenetic analysis
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - blood
Sequence Analysis, DNA
Viral diseases
Viral hepatitis
Viral Proteins - genetics
Virology
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Summary:To determine hepatitis C virus (HCV) genotype distribution in China, a total of 148 HCV RNA positive serum samples were collected from nine geographic areas and subjected to RT‐PCR followed by direct DNA sequencing and phylogenetic analysis of the core, E1, and NS5B regions. HCV was genotyped in 139 (93.9%) samples. Among them subtype 1b was the most predominant [66% (92/139)] followed by 2a [14% (19/139)]. Of 92 subtype 1b isolates, 35 (38%) and 30 (33%) formed two clusters, designated groups A and B. Group A was prevalent throughout China, while group B was predominant in the central and southern regions. In three cities in the Pearl River Delta, subtype 6a replaced 2a as the second most predominant subtype, and in Kunming (southwest) multiple HCV genotypes/subtypes were present. New variants of HCV genotype 6 were discovered in three samples from Kunming and one in Guangzhou in the Pearl River Delta. J. Med. Virol. 75:538–549, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.20307