TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants

Nuclear matrix attachment regions (MARs) are thought to influence the expression of the flanking genes. TM2, a new DNA fragment isolated from tobacco, can bind with the rice nuclear matrix in vitro. In this study, we investigated the effect of TM2 on transgene expression under the control of three d...

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Bibliographic Details
Published in:Theoretical and applied genetics 2005-02, Vol.110 (4), p.620-627
Main Authors: Xue, H, Yang, Y.T, Wu, C.A, Yang, G.D, Zhang, M.M, Zheng, C.C
Format: Article
Language:English
Subjects:
Base Sequence
beta-glucuronidase
Biological and medical sciences
callus
callus culture
Classical genetics, quantitative genetics, hybrids
DNA
DNA, Plant - genetics
DNA, Plant - isolation & purification
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Plant
gene transfer
Genetics of eukaryotes. Biological and molecular evolution
Glucuronidase - genetics
Glucuronidase - metabolism
matrix attachment region
Molecular Sequence Data
Nicotiana - cytology
Nicotiana - genetics
Nicotiana tabacum
Nuclear Matrix
Oryza - genetics
Oryza sativa
Plants, Genetically Modified
Promoter Regions, Genetic
Pteridophyta, spermatophyta
reporter genes
rice
tobacco
Transformation, Genetic
Transgenes
transgenic plants
Ubiquitin - genetics
Vegetals
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Summary:Nuclear matrix attachment regions (MARs) are thought to influence the expression of the flanking genes. TM2, a new DNA fragment isolated from tobacco, can bind with the rice nuclear matrix in vitro. In this study, we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants. The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants. The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic tissues of transformants. However, TM2 did not change the gene expression pattern controlled by the PNZIP promoter. The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters. Our results indicate that TM2, as a novel strong MAR, can be used to increase the transgene expression levels in the whole plant or in particular tissues of monocotyledons.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-004-1880-9