Autologous transplantation of porcine myogenic precursor cells in skeletal muscle

Myoblast transplantation is a potential therapy for severe muscle trauma, myopathies and heart infarct. Success with this therapy relies on the ability to obtain cell preparations enriched in myogenic precursor cells and on their survival after transplantation. To define myoblast transplantation str...

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Bibliographic Details
Published in:Neuromuscular disorders : NMD 2005-03, Vol.15 (3), p.237-244
Main Authors: Holzer, Nicolas, Hogendoorn, Simone, Zürcher, Line, Garavaglia, Guido, Yang, Sheng, König, Stephane, Laumonier, Thomas, Menetrey, Jacques
Format: Article
Language:English
Subjects:
Animals
CD56 Antigen - metabolism
Cell Count - methods
Cell Proliferation
Cell survival
Cell Survival - physiology
Desmin - metabolism
Female
Flow Cytometry
Indoles
Muscle Development
Muscle, Skeletal - physiology
Muscle, Skeletal - transplantation
Myoblasts - physiology
Myoblasts - transplantation
Myogenic precursor cell
Pig
Swine
Thymidine - metabolism
Time Factors
Transduction, Genetic
Transplantation
Transplantation, Autologous
Tritium - metabolism
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Summary:Myoblast transplantation is a potential therapy for severe muscle trauma, myopathies and heart infarct. Success with this therapy relies on the ability to obtain cell preparations enriched in myogenic precursor cells and on their survival after transplantation. To define myoblast transplantation strategies applicable to patients, we used a large animal model, the pig. Muscle dissociation procedures adapted to porcine tissue gave high yields of cells containing at least 80% myogenic precursor cells. Autologous transplantation of 3[H]-thymidine labeled porcine myogenic precursor cells indicated 60% survival at day 1 followed by a decay to 10% at day 5 post-injection. Nuclei of myogenic precursor cells transduced with a lentivirus encoding the nls-lacZ reporter gene were present in host myotubes 8 days post-transplantation, indicating that injected myogenic precursor cells contribute to muscle regeneration. This work suggests that pig is an adequate large animal model for exploring myogenic precursor cells transplantation strategies applicable in patients.
ISSN:0960-8966
1873-2364
DOI:10.1016/j.nmd.2004.11.001