Cloning of the Atlantic salmon ( Salmo salar) IL-1 receptor associated protein

In mammals, the pro-inflammatory cytokine interleukin-1 signals through a receptor complex containing a type I interleukin-1 receptor (IL-1RI) and a receptor associated protein (IL-1RAcP). Previously, we have described a cDNA from Atlantic salmon encoding a molecule with homology to the mammalian IL...

Full description

Saved in:
Bibliographic Details
Published in:Fish & shellfish immunology 2005-07, Vol.19 (1), p.53-65
Main Authors: Stansberg, Christine, Subramaniam, Sumathi, Collet, Bertrand, Secombes, Christopher J., Cunningham, Charles
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cercopithecus aethiops
Cloning, Molecular
Cluster Analysis
COS Cells
DNA Primers
Gene Library
IL-1
IL-1R family
IL-1RAcP
IL-1RLP
Immunoprecipitation
Marine
Membrane Proteins - genetics
Membrane Proteins - metabolism
Molecular Sequence Data
Phylogeny
Protein Structure, Tertiary
Receptors, Interleukin-1 - genetics
Receptors, Interleukin-1 - metabolism
Salmo salar
Salmo salar - genetics
Sequence Alignment
Sequence Analysis, DNA
Signal Transduction - immunology
TIR family
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In mammals, the pro-inflammatory cytokine interleukin-1 signals through a receptor complex containing a type I interleukin-1 receptor (IL-1RI) and a receptor associated protein (IL-1RAcP). Previously, we have described a cDNA from Atlantic salmon encoding a molecule with homology to the mammalian IL-RI. This molecule was named IL-1 receptor like protein (IL-1RLP) in the absence of functional data to support its proposed role as the salmon IL-1RI. Here, we describe the cloning and characterisation of a cDNA encoding salmon IL-1RAcP. Like other members of the IL-1R family, the salmon IL-1RAcP encodes three extracellular immunoglobulin-like domains and a cytoplasmic Toll/Interleukin-1 receptor (TIR) domain involved in signalling. Specific binding of salmon IL-1RAcP to IL-1RLP was shown by co-immunoprecipitation studies.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2004.11.006