Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells

We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene ( PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 invol...

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Bibliographic Details
Published in:Blood cells, molecules, & diseases molecules, & diseases, 2005-03, Vol.34 (2), p.186-190
Main Authors: de Vooght, Karen M.K., van Wijk, Richard, van Oirschot, Brigitte A., Rijksen, Gert, van Solinge, Wouter W.
Format: Article
Language:English
Subjects:
Base Sequence
DNA Primers
Erythroid-specific gene expression
Gene Expression Regulation, Neoplastic
Hexokinase - genetics
Hexokinase gene expression
HK1
Humans
K562 Cells
PKLR
PKR-RE1
Promoter Regions, Genetic
Regulatory Sequences, Ribonucleic Acid
Transcriptional regulation
Transfection
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Summary:We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene ( PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene ( HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the PKLR and HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression.
ISSN:1079-9796
1096-0961
DOI:10.1016/j.bcmd.2004.10.002