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Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells
We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene ( PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 invol...
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| Published in: | Blood cells, molecules, & diseases molecules, & diseases, 2005-03, Vol.34 (2), p.186-190 |
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| Main Authors: | , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c354t-dd6015316c6e2f62fa4ea30e4505f34d037f3859029318ab3a8a2151702911753 |
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| cites | cdi_FETCH-LOGICAL-c354t-dd6015316c6e2f62fa4ea30e4505f34d037f3859029318ab3a8a2151702911753 |
| container_end_page | 190 |
| container_issue | 2 |
| container_start_page | 186 |
| container_title | Blood cells, molecules, & diseases |
| container_volume | 34 |
| creator | de Vooght, Karen M.K. van Wijk, Richard van Oirschot, Brigitte A. Rijksen, Gert van Solinge, Wouter W. |
| description | We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene (
PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene (
HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the
PKLR and
HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression. |
| doi_str_mv | 10.1016/j.bcmd.2004.10.002 |
| format | article |
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PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene (
HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the
PKLR and
HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression.</description><identifier>ISSN: 1079-9796</identifier><identifier>EISSN: 1096-0961</identifier><identifier>DOI: 10.1016/j.bcmd.2004.10.002</identifier><identifier>PMID: 15727904</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Base Sequence ; DNA Primers ; Erythroid-specific gene expression ; Gene Expression Regulation, Neoplastic ; Hexokinase - genetics ; Hexokinase gene expression ; HK1 ; Humans ; K562 Cells ; PKLR ; PKR-RE1 ; Promoter Regions, Genetic ; Regulatory Sequences, Ribonucleic Acid ; Transcriptional regulation ; Transfection</subject><ispartof>Blood cells, molecules, & diseases, 2005-03, Vol.34 (2), p.186-190</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-dd6015316c6e2f62fa4ea30e4505f34d037f3859029318ab3a8a2151702911753</citedby><cites>FETCH-LOGICAL-c354t-dd6015316c6e2f62fa4ea30e4505f34d037f3859029318ab3a8a2151702911753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15727904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Vooght, Karen M.K.</creatorcontrib><creatorcontrib>van Wijk, Richard</creatorcontrib><creatorcontrib>van Oirschot, Brigitte A.</creatorcontrib><creatorcontrib>Rijksen, Gert</creatorcontrib><creatorcontrib>van Solinge, Wouter W.</creatorcontrib><title>Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells</title><title>Blood cells, molecules, & diseases</title><addtitle>Blood Cells Mol Dis</addtitle><description>We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene (
PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene (
HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the
PKLR and
HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression.</description><subject>Base Sequence</subject><subject>DNA Primers</subject><subject>Erythroid-specific gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hexokinase - genetics</subject><subject>Hexokinase gene expression</subject><subject>HK1</subject><subject>Humans</subject><subject>K562 Cells</subject><subject>PKLR</subject><subject>PKR-RE1</subject><subject>Promoter Regions, Genetic</subject><subject>Regulatory Sequences, Ribonucleic Acid</subject><subject>Transcriptional regulation</subject><subject>Transfection</subject><issn>1079-9796</issn><issn>1096-0961</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp9kEtv2zAQhImiRfNo_0APAU9Fc5C7S4qUBPRSGM4DCdDAaM4MLa0cuno4pGTE_74UbCC3HBZcDGYGy4-xbwgzBNQ_N7NV2VYzAZBGYQYgPrBThEIncfDjtGdFUmSFPmFnIWwAALHIP7MTVJnICkhP2dPD3o87OxD_5zobiHtaj40der_n1FBL3cCR_3i4WybLBV7ylioX3YE_02t_jKypI06vW08huL7jruN3SgteUtOEL-xTbZtAX4_vOXu8Wvyd3yT3f65v57_vk1KqdEiqSgMqibrUJGotapuSlUCpAlXLtAKZ1TJXBYhCYm5X0uZWoMIsCoiZkufs-6F36_uXkcJgWhemC2xH_RiMzlIlc8ijURyMpe9D8FSbrXet9XuDYCauZmMmrmbiOmmRawxdHNvHVUTwFjmCjIZfBwPFP-4ceRNKR10ZcXkqB1P17r3-_xGvhuM</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>de Vooght, Karen M.K.</creator><creator>van Wijk, Richard</creator><creator>van Oirschot, Brigitte A.</creator><creator>Rijksen, Gert</creator><creator>van Solinge, Wouter W.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050301</creationdate><title>Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells</title><author>de Vooght, Karen M.K. ; van Wijk, Richard ; van Oirschot, Brigitte A. ; Rijksen, Gert ; van Solinge, Wouter W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-dd6015316c6e2f62fa4ea30e4505f34d037f3859029318ab3a8a2151702911753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>DNA Primers</topic><topic>Erythroid-specific gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hexokinase - genetics</topic><topic>Hexokinase gene expression</topic><topic>HK1</topic><topic>Humans</topic><topic>K562 Cells</topic><topic>PKLR</topic><topic>PKR-RE1</topic><topic>Promoter Regions, Genetic</topic><topic>Regulatory Sequences, Ribonucleic Acid</topic><topic>Transcriptional regulation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Vooght, Karen M.K.</creatorcontrib><creatorcontrib>van Wijk, Richard</creatorcontrib><creatorcontrib>van Oirschot, Brigitte A.</creatorcontrib><creatorcontrib>Rijksen, Gert</creatorcontrib><creatorcontrib>van Solinge, Wouter W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood cells, molecules, & diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Vooght, Karen M.K.</au><au>van Wijk, Richard</au><au>van Oirschot, Brigitte A.</au><au>Rijksen, Gert</au><au>van Solinge, Wouter W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells</atitle><jtitle>Blood cells, molecules, & diseases</jtitle><addtitle>Blood Cells Mol Dis</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>34</volume><issue>2</issue><spage>186</spage><epage>190</epage><pages>186-190</pages><issn>1079-9796</issn><eissn>1096-0961</eissn><abstract>We have established the functional importance of PKR-RE1, a necessary transcriptional regulatory element in the erythroid-specific promoter of the human pyruvate kinase gene (
PKLR). Here, we demonstrate by electrophoretic mobility shift assay (EMSA) that the DNA–protein interaction at PKR-RE1 involves a CTGTC motif. Because the same motif is also present in the erythroid-specific promoter of the hexokinase gene (
HK1), we confirmed its functional relevance by in vitro transfection in K562 cells. Moreover, EMSA demonstrated that the CTGTC motif in both the
PKLR and
HK1 promoters mediates binding of the same protein. Therefore, we postulate a more general role of PKR-RE1 in erythroid-specific gene expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15727904</pmid><doi>10.1016/j.bcmd.2004.10.002</doi><tpages>5</tpages></addata></record> |
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| ispartof | Blood cells, molecules, & diseases, 2005-03, Vol.34 (2), p.186-190 |
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| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Base Sequence DNA Primers Erythroid-specific gene expression Gene Expression Regulation, Neoplastic Hexokinase - genetics Hexokinase gene expression HK1 Humans K562 Cells PKLR PKR-RE1 Promoter Regions, Genetic Regulatory Sequences, Ribonucleic Acid Transcriptional regulation Transfection |
| title | Pyruvate kinase regulatory element 1 (PKR-RE1) mediates hexokinase gene expression in K562 cells |
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