Analysis of hydrophilic metabolites in physiological fluids by HPLC-MS using a silica hydride-based stationary phase

Aqueous normal-phase chromatography was used for the analysis of metabolites in human saliva and urine samples. The column was packed with a silica hydride type separation material. Several gradients were tested with different mobile phase additives in order to produce retention for amino acids, sma...

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Bibliographic Details
Published in:Journal of separation science 2009-07, Vol.32 (13), p.2200-2208
Main Authors: Pesek, Joseph J, Matyska, Maria T, Loo, Joseph A, Fischer, Steven M, Sana, Theodore R
Format: Article
Language:English
Subjects:
Aqueous normal-phase
Biological and medical sciences
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Creatinine - analysis
Digestive system
Gastroenterology. Liver. Pancreas. Abdomen
Glucose - analysis
Glutamine - analysis
Host-tumor relations. Immunology. Biological markers
Humans
Hydrophobic and Hydrophilic Interactions
Investigative techniques, diagnostic techniques (general aspects)
LC-MS
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Lysine - analysis
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Medical sciences
Metabolomics
Pancreatic Neoplasms - chemistry
Pancreatic Neoplasms - urine
Pancreatitis - urine
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polar analytes
Saliva
Saliva - chemistry
Silicates - chemistry
Tumors
Urine
Urine - chemistry
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Summary:Aqueous normal-phase chromatography was used for the analysis of metabolites in human saliva and urine samples. The column was packed with a silica hydride type separation material. Several gradients were tested with different mobile phase additives in order to produce retention for amino acids, small organic acids, and carbohydrates. Detection was done by TOF MS. In some cases the relative concentration levels of various metabolites in human saliva were compared for normal patients and patients with pancreatic cancer or pancreatitis. The reproducibility of retention of individual metabolites in these complex matrices was tested for several compounds.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900270