Identification of novel QTLs for seedling and adult plant leaf rust resistance in a wheat doubled haploid population

• Published in: Theoretical and applied genetics 2009-07, Vol.119 (2), p.263-269
• Main Authors: Chu, C.-G, Friesen, T. L, Xu, S. S, Faris, J. D, Kolmer, J. A
• Format: Article
• Language: English
• Subjects: Agriculture; Basidiomycota - physiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; chromosome mapping; Chromosomes; Classical genetics, quantitative genetics, hybrids; Cultivars; disease resistance; doubled haploids; Fundamental and applied biological sciences. Psychology; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Haploidy; hard red spring wheat; Immunity, Innate - genetics; Infections; Life Sciences; mature plants; Original Paper; Physical Chromosome Mapping; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant Leaves - genetics; Plant Leaves - microbiology; Plant resistance; Pteridophyta, spermatophyta; Puccinia recondita; quantitative trait loci; Quantitative Trait Loci - genetics; rust diseases; seedling diseases; seedlings; Seedlings - genetics; Seedlings - microbiology; Triticum - genetics; Triticum - microbiology; Triticum aestivum; Vegetals; wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-009-1035-0

Pyramiding of genes that confer partial resistance is a method for developing wheat (Triticum aestivum L.) cultivars with durable resistance to leaf rust caused by Puccinia triticina. In this research, a doubled haploid population derived from the cross between the synthetic hexaploid wheat (SHW) (xAegilotriticum spp.) line TA4152-60 and the North Dakota breeding line ND495 was used for identifying genes conferring partial resistance to leaf rust in both the adult plant and seedling stages. Five QTLs located on chromosome arms 3AL, 3BL, 4DL, 5BL and 6BL were associated with adult plant resistance with the latter four representing novel leaf rust resistance QTLs. Resistance effects of the 4DL QTL were contributed by ND495 and the effects of the other QTLs were contributed by the SHW line. The QTL on chromosome arm 3AL had large effects and also conferred seedling resistance to leaf rust races MJBJ, TDBG and MFPS. The other major QTL, which was on chromosome arm 3BL, conferred seedling resistance to race MFPS and was involved in a significant interaction with a locus on chromosome arm 5DS. The QTLs and the associated molecular markers identified in this research can be used to develop wheat cultivars with potentially durable leaf rust resistance.