IgH V-Region Sequence Does Not Predict the Survival Fate of Human Germinal Center B Cells

Germinal center (GC) B cell survival fate is governed in part by the outcome of successful/failed BCR-mediated interactions with accessory cells. However, the extent to which the BCR primary sequence influences such interactions is not fully understood. Over 1000 IgV(H)4 family cDNAs were sequenced...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2005-03, Vol.174 (5), p.2805-2813
Main Authors: Jackson, Stephen M, Capra, J. Donald
Format: Article
Language:English
Subjects:
Annexin A5 - biosynthesis
Apoptosis - genetics
Apoptosis - immunology
B-Lymphocyte Subsets - cytology
B-Lymphocyte Subsets - immunology
B-Lymphocyte Subsets - metabolism
Cell Survival - genetics
Cell Survival - immunology
Complementarity Determining Regions - biosynthesis
Complementarity Determining Regions - genetics
Gene Frequency
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Germinal Center - cytology
Germinal Center - immunology
Germinal Center - metabolism
Humans
Immunoglobulin G - genetics
Immunoglobulin Heavy Chains - biosynthesis
Immunoglobulin Heavy Chains - genetics
Immunoglobulin Heavy Chains - metabolism
Immunoglobulin Joining Region - genetics
Immunoglobulin Variable Region - biosynthesis
Immunoglobulin Variable Region - genetics
Immunoglobulin Variable Region - metabolism
Peptide Fragments - biosynthesis
Peptide Fragments - genetics
Phagocytosis - genetics
Phagocytosis - immunology
Receptors, Antigen, B-Cell - metabolism
Sequence Analysis, DNA
Somatic Hypermutation, Immunoglobulin
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Summary:Germinal center (GC) B cell survival fate is governed in part by the outcome of successful/failed BCR-mediated interactions with accessory cells. However, the extent to which the BCR primary sequence influences such interactions is not fully understood. Over 1000 IgV(H)4 family cDNAs were sequenced from living (annexin V(-)) and apoptotic (annexin V(+) or from within tingible body macrophages) GC B cell fractions from seven tonsils. Results surprisingly demonstrate that living and dying GC B cells do not significantly differ in IgV(H), D, or J(H) gene segment use; HCDR3 length or positive charge; or mutation frequency. Additionally, equivalent IgH cDNA sequences were identified in both fractions, suggesting that BCR sequence alone is an unreliable predictor of GC B cell survival.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.174.5.2805