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Proteomic and transcriptomic characterization of interferon-α-induced human primary T helper cells

Interferon‐α (IFN‐α) is a multifunctional cytokine that modulates immune response. In spite of the numerous comprehensive studies on the effects of IFN‐α on various cell types, novel characteristics of this versatile agent emerge continuously. In the present study a differential proteomic approach w...

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Published in:Proteomics (Weinheim) 2005-02, Vol.5 (2), p.371-379
Main Authors: Rosengren, Arsi T., Nyman, Tuula A., Syyrakki, Saija, Matikainen, Sampsa, Lahesmaa, Riitta
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container_title Proteomics (Weinheim)
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creator Rosengren, Arsi T.
Nyman, Tuula A.
Syyrakki, Saija
Matikainen, Sampsa
Lahesmaa, Riitta
description Interferon‐α (IFN‐α) is a multifunctional cytokine that modulates immune response. In spite of the numerous comprehensive studies on the effects of IFN‐α on various cell types, novel characteristics of this versatile agent emerge continuously. In the present study a differential proteomic approach was used to identify new IFN‐α‐regulated proteins in human primary CD4+ T cells. Two IFN‐α‐inducible proteins, soluble N‐ethylmaleimide‐sensitive factor attachment protein &α; (&α;‐SNAP) and cleavage stimulation factor‐64 (CstF‐64) previously not described in this context, were identified. Additionally, several proteins already known as IFN‐stimulated genes were observed. The results of proteomics experiments were further studied at the mRNA level using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). Both peripheral blood and cord blood CD4+ T cells were used in order to see if there are differences in IFN‐α response between these populations. Differences were observed between the IFN‐α‐induced expression kinetics in peripheral blood and cord blood transcripts. The induction was more rapid in peripheral blood than in cord blood cells. CstF‐64 expression was upregulated by IFN‐α at the protein, but not at the mRNA level.
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source Wiley-Blackwell Read & Publish Collection
subjects Analytical, structural and metabolic biochemistry
Autoradiography
Biological and medical sciences
CD4+ T cell
CD4-Positive T-Lymphocytes - immunology
Cells, Cultured
Differential proteomics
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fetal Blood - immunology
Fundamental and applied biological sciences. Psychology
Humans
Infant, Newborn
Interferon-alpha - genetics
Interferon-alpha - pharmacology
Interferon-gamma - biosynthesis
Interferon-α
Kinetics
Leukocytes, Mononuclear - immunology
Lymphocyte Activation
Miscellaneous
Peptide Mapping
Proteins
Proteomics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Silver Staining
Solubility
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
T-Lymphocytes, Helper-Inducer - drug effects
T-Lymphocytes, Helper-Inducer - immunology
T-Lymphocytes, Helper-Inducer - metabolism
Transcription, Genetic
Two-dimensional gel electrophoresis
Vesicular Transport Proteins - chemistry
Vesicular Transport Proteins - metabolism
title Proteomic and transcriptomic characterization of interferon-α-induced human primary T helper cells
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