Direct Antigen Presentation and Gap Junction Mediated Cross-Presentation during Apoptosis

MHC class I molecules present peptides from endogenous proteins. Ags can also be presented when derived from extracellular sources in the form of apoptotic bodies. Cross-presentation of such Ags by dendritic cells is required for proper CTL responses. The fate of Ags in cells initiated for apoptosis...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2009-07, Vol.183 (2), p.1083-1090
Main Authors: Pang, Baoxu, Neijssen, Joost, Qiao, Xiaohang, Janssen, Lennert, Janssen, Hans, Lippuner, Christoph, Neefjes, Jacques
Format: Article
Language:English
Subjects:
Antigen Presentation - immunology
Apoptosis - immunology
Caspase 9 - metabolism
Caspases - metabolism
Cell Line
Coculture Techniques
Connexin 43
Cross-Priming
Dendritic Cells - immunology
Gap Junctions - immunology
Histocompatibility Antigens Class I - immunology
Humans
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Summary:MHC class I molecules present peptides from endogenous proteins. Ags can also be presented when derived from extracellular sources in the form of apoptotic bodies. Cross-presentation of such Ags by dendritic cells is required for proper CTL responses. The fate of Ags in cells initiated for apoptosis is unclear as is the mechanism of apoptosis-derived Ag transfer into dendritic cells. Here we show that novel Ags can be generated by caspases and be presented by MHC class I molecules of apoptotic cells. Since gap junctions function until apoptotic cells remodel to form apoptotic bodies, transfer and cross-presentation of apoptotic peptides by neighboring and dendritic cells occurs. We thus define a novel phase in classical Ag presentation and cross-presentation by MHC class I molecules: presentation of Ags created by caspase activities in cells in apoptosis.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.0900861