Antibody processing and engineering in plants, and new strategies for vaccine production

The use of transgenic plants for the production of recombinant proteins is not a universal solution for all proteins. The choice of this expression system depends very much on the type of protein and its applications. Many proteins will best be made by conventional microbial fermentation, similarly,...

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Bibliographic Details
Published in:Vaccine 2005-03, Vol.23 (15), p.1814-1818
Main Authors: Ma, Julian K-C., Drake, Pascal M.W., Chargelegue, Daniel, Obregon, Patricia, Prada, Alessandra
Format: Article
Language:English
Subjects:
Animals
Antibodies - metabolism
Antibody
Antibody Formation
Applied microbiology
Biological and medical sciences
Chaperones
Endoplasmic reticulum
Enzymes
Fermentation
Fundamental and applied biological sciences. Psychology
Genes
Glycosylation
Humans
Light
Mammals
Microbiology
Plants
Plants, Genetically Modified - genetics
Plants, Genetically Modified - immunology
Protein Engineering
Protein folding
Proteins
Secretory pathway
Seeds
Studies
Transgenic plants
Vaccines - biosynthesis
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:The use of transgenic plants for the production of recombinant proteins is not a universal solution for all proteins. The choice of this expression system depends very much on the type of protein and its applications. Many proteins will best be made by conventional microbial fermentation, similarly, we are already identifying proteins where plants represent the only practical option for one reason or another. It will be important to understand better the cellular mechanisms of protein folding, assembly and processing in plants, in order to maximise the potential of transgenic plants as a protein production system. One of the main advantages that plants offer is that they are higher eukaryotic organisms with an endomembrane system. Therefore, they fold and assemble recombinant proteins using protein chaperones that are homologous to those in mammalian cells, and they perform post-translational modifications. This allows, for example, the expression of monoclonal antibodies, first described in 1989, as well as a range of other types of immunoglobulin molecules and multimeric complexes.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2004.11.011