Selective labeling of tag-fused protein by tryptophan-sensitized luminescence of a terbium complex

A Tb3+ complex with two di(2-picolyl)amine (DPA) moieties and an oligo-aspartate peptide containing a tryptophan residue have been designed as a new binding pair for use in protein labeling.

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2009-01 (22), p.3196-3198
Main Authors: Hirayama, Tasuku, Taki, Masayasu, Kodan, Atsushi, Kato, Hiroaki, Yamamoto, Yukio
Format: Article
Language:English
Subjects:
Amines - chemistry
Amino Acid Sequence
Luminescent Measurements
Organometallic Compounds - chemistry
Peptides - chemistry
Picolinic Acids - chemistry
Proteins - analysis
Proteins - chemistry
Staining and Labeling
Substrate Specificity
Terbium - chemistry
Tryptophan
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Description
Summary:A Tb3+ complex with two di(2-picolyl)amine (DPA) moieties and an oligo-aspartate peptide containing a tryptophan residue have been designed as a new binding pair for use in protein labeling.
ISSN:1359-7345
1364-548X
DOI:10.1039/b900302a