Blood treatment influences the yield of apoptotic lymphocytes after maximal exercise

No systematic investigation has been reported assessing the effect of cell isolation processes on postexercise apoptosis. Therefore, the effect of cell isolation procedures on apoptosis was evaluated in this study. Untrained healthy individuals participated (N=13). Blood samples obtained at rest and...

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Bibliographic Details
Published in:Medicine and science in sports and exercise 2005-03, Vol.37 (3), p.369-373
Main Authors: NAVALTA, James W, SEDLOCK, Darlene A, PARK, Kyung-Shin
Format: Article
Language:English
Subjects:
Adult
Apoptosis - physiology
Biological and medical sciences
Cell Separation - methods
Exercise - physiology
Female
Fundamental and applied biological sciences. Psychology
Humans
Lymphocyte Count - methods
Lymphocytes - cytology
Male
Physical Endurance - physiology
Physical Exertion - physiology
Reference Values
Rest - physiology
Space life sciences
Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports
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Summary:No systematic investigation has been reported assessing the effect of cell isolation processes on postexercise apoptosis. Therefore, the effect of cell isolation procedures on apoptosis was evaluated in this study. Untrained healthy individuals participated (N=13). Blood samples obtained at rest and immediately after an incremental exercise test to exhaustion were partitioned into three treatments: 1) whole blood smears made immediately after the sample was obtained (WB), 2) cells subjected to density-gradient isolation before smears were made (ISO), and 3) samples allowed to sit at room temperature (i.e., time-treated) before centrifugation and smearing (TT). Blood smears were stained using the May-Grünwald Giemsa procedure and lymphocytes were evaluated under a light microscope for characteristic features of apoptosis. Data were analyzed using a 2x3 ANOVA. A significant interaction effect existed (P
ISSN:0195-9131
1530-0315
DOI:10.1249/01.MSS.0000155433.08698.C1