Narrow-bore high performance liquid chromatographic method for the determination of cetirizine in human plasma using column switching

An improved column switching high performance liquid chromatographic (HPLC) method was developed for determination of cetirizine in human plasma. Plasma samples were prepared by liquid–liquid extraction using methylene chloride. The samples extracted were initially injected into a clean-up Capcell P...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2005-03, Vol.37 (3), p.603-609
Main Authors: Kim, Chong-Kook, Yeon, Kyu Jeong, Ban, Eunmi, Hyun, Myung-Ja, Kim, Jin-Ki, Kim, Mee-Kyung, Jin, Su-Eon, Park, Jeong-Sook
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cetirizine
Cetirizine - administration & dosage
Cetirizine - blood
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Column switching
Fundamental and applied biological sciences. Psychology
General pharmacology
High performance liquid chromatography (HPLC)
Humans
Medical sciences
Pharmacokinetics
Pharmacology. Drug treatments
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Summary:An improved column switching high performance liquid chromatographic (HPLC) method was developed for determination of cetirizine in human plasma. Plasma samples were prepared by liquid–liquid extraction using methylene chloride. The samples extracted were initially injected into a clean-up Capcell Pak MF C 8 column and the peaks of cetirizine and internal standard were separated to an analytical C 18 micro-column via column switching device. This analysis showed highly sensitive and selective results. Also, it was successfully applied to evaluate the pharmacokinetics of cetirizine in human volunteers after single oral administration.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2004.11.022