Methyl Esterification Divergently Affects the Degradability of Pectic Uronosyls in Nonlignified and Lignified Maize Cell Walls

Nonlignified cell walls from Zea mays (L.) cell suspensions were incubated with and without pectin methylesterase (PME) and a portion were artificially lignified to assess how methyl esters influence the release of pectic uronosyls and total sugars from cell walls by fungal enzymes. Treatment with P...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-03, Vol.53 (5), p.1546-1549
Main Authors: Grabber, John H, Hatfield, Ronald D
Format: Article
Language:English
Subjects:
artificial lignification
Biological and medical sciences
Carboxylic Ester Hydrolases - metabolism
cell suspension culture
Cell Wall - chemistry
cell walls
Cereal and baking product industries
corn
enzymatic hydrolysis
Esterification
Food industries
Fundamental and applied biological sciences. Psychology
Hexuronic Acids - chemistry
Hexuronic Acids - metabolism
lignification
Lignin - analysis
Lignin - chemistry
methylated uronic acids
Methylation
nonlignified cell walls
pectinesterase
pectins
uronic acids
Uronic Acids - metabolism
Zea mays
Zea mays - ultrastructure
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Summary:Nonlignified cell walls from Zea mays (L.) cell suspensions were incubated with and without pectin methylesterase (PME) and a portion were artificially lignified to assess how methyl esters influence the release of pectic uronosyls and total sugars from cell walls by fungal enzymes. Treatment with PME reduced uronosyl concentrations from 97 to 92 mg/g, reduced uronosyl methylation from 57% to 21%, and increased Klason lignin concentrations in artificially lignified cell walls from 99 to 116 mg/g. Although PME treatment slightly enhanced uronosyl release from nonlignified cell walls, it reduced uronosyl release from artificially lignified cell walls by 55% after 4 h and by 7% after 72 h of enzymatic hydrolysis. Pectin hydrolysis in PME treated cell walls was probably impaired by enhanced benzyl ester cross-linking of uronosyls to lignin via quinone methide intermediates. Variations in uronosyl methylation had little effect on the overall release of total sugars from cell walls. Keywords: Zea mays; quinone methide intermediate; benzyl ester; cross-link; polygalacturonic acid; pectin; lignin; degradability; digestibility; pectinase; polygalacturonase
ISSN:0021-8561
1520-5118
DOI:10.1021/jf048799b