Time-Resolved Tracking of a Minimum Gene Expression System Reconstituted in Giant Liposomes

Individual expression: We describe a method that allows the observation of real-time gene expression in a large number of individual giant liposomes encapsulating identical genetic material. We followed the gene expression profiles from DNA and mRNA templates coding for different proteins. Although...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2009-07, Vol.10 (10), p.1640-1643
Main Authors: Saito, Hirohide, Kato, Yusho, Le Berre, Maël, Yamada, Ayako, Inoue, Tan, Yosikawa, Kenichi, Baigl, Damien
Format: Article
Language:English
Subjects:
DNA
DNA - metabolism
Gene Expression
Gene Expression Profiling
GFP expression
giant liposomes
Green Fluorescent Proteins - genetics
Liposomes - chemistry
Liposomes - pharmacology
RNA
RNA, Messenger - metabolism
Time Factors
translation
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Summary:Individual expression: We describe a method that allows the observation of real-time gene expression in a large number of individual giant liposomes encapsulating identical genetic material. We followed the gene expression profiles from DNA and mRNA templates coding for different proteins. Although the average profiles of individual liposomes were similar to those measured in bulk solution, strong variability between individual liposomes was observed at both transcription and translation.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200900205