Genetic Discovery in Xylella fastidiosa Through Sequence Analysis of Selected Randomly Amplified Polymorphic DNAs

Xylella fastidiosa causes many important plant diseases including Pierce's disease (PD) in grape and almond leaf scorch disease (ALSD). DNA-based methodologies, such as randomly amplified polymorphic DNA (RAPD) analysis, have been playing key roles in genetic information collection of the bacte...

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Bibliographic Details
Published in:Current microbiology 2005-02, Vol.50 (2), p.78-83
Main Authors: Chen, J, Civerolo, E.L, Jarret, R.L, Van Sluys, M.A, Oliveira, M.C. de
Format: Article
Language:English
Subjects:
Bacteria
Bacteriophages - genetics
Bacteriophages - isolation & purification
Base Sequence
Conjugation, Genetic
Data collection
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Evolution, Molecular
Gene Rearrangement
Genes, Bacterial
Genetics
Genome, Bacterial
Genomics
Molecular Sequence Data
Pierce's disease
Plant diseases
Plant Diseases - microbiology
plant pathogenic bacteria
plasmids
Plasmids - genetics
Podoviridae - genetics
Podoviridae - isolation & purification
Polymorphism, Single Nucleotide
Random Amplified Polymorphic DNA Technique
sequence analysis
Xylella - genetics
Xylella - pathogenicity
Xylella - virology
Xylella fastidiosa
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Summary:Xylella fastidiosa causes many important plant diseases including Pierce's disease (PD) in grape and almond leaf scorch disease (ALSD). DNA-based methodologies, such as randomly amplified polymorphic DNA (RAPD) analysis, have been playing key roles in genetic information collection of the bacterium. This study further analyzed the nucleotide sequences of selected RAPDs from X. fastidiosa strains in conjunction with the available genome sequence databases and unveiled several previously unknown novel genetic traits. These include a sequence highly similar to those in the phage family of Podoviridae. Genome comparisons among X. fastidiosa strains suggested that the "phage" is currently active. Two other RAPDs were also related to horizontal gene transfer: one was part of a broadly distributed cryptic plasmid and the other was associated with conjugal transfer. One RAPD inferred a genomic rearrangement event among X. fastidiosa PD strains and another identified a single nucleotide polymorphism of evolutionary value.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-004-4412-6