Use of RNA Interference to Prevent Lethal Murine West Nile Virus Infection

West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge wit...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-04, Vol.191 (7), p.1148-1154
Main Authors: Bai, Fengwei, Wang, Tian, Pal, Utpal, Bao, Fukai, Gould, L. Hannah, Fikrig, Erol
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cercopithecus aethiops
Disease Models, Animal
Female
Fundamental and applied biological sciences. Psychology
Genes, Viral
Infections
Infectious diseases
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
RNA
RNA Interference
RNA, Small Interfering - therapeutic use
Small interfering RNA
Survival Analysis
Transfection
Vero Cells
Viral Load
Virology
Viruses
West Nile Fever - prevention & control
West Nile Fever - virology
West Nile virus
West Nile virus - physiology
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Summary:West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge with an intraperitoneal inoculum of West Nile virus reduced the viral load and afforded partial protection from lethal infection. These data show the efficacy of the prophylactic use of siRNAs against a viral infection in vivo and suggest new strategies to combat West Nile virus
ISSN:0022-1899
1537-6613
DOI:10.1086/428507