Intracellular persistence of Escherichia coli in urinary bladders from mecillinam-treated mice

Objectives: It has been suggested recently that intracellular bacteria surviving antibiotic treatment might serve as a reservoir for recurrent infection. The purpose of this study was to directly examine the location of Escherichia coli bacteria in the mouse bladder after treatment with mecillinam....

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2005-03, Vol.55 (3), p.383-386
Main Authors: Kerrn, M. B., Struve, C., Blom, J., Frimodt-Møller, N., Krogfelt, K. A.
Format: Article
Language:English
Subjects:
Amdinocillin - pharmacology
Animals
Anti-Infective Agents, Urinary - pharmacology
antibiotic treatments
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Colony Count, Microbial
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - growth & development
Humans
Medical sciences
Mice
Microscopy, Electron
mouse models
Pharmacology. Drug treatments
Urinary Bladder - microbiology
UTIs
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Summary:Objectives: It has been suggested recently that intracellular bacteria surviving antibiotic treatment might serve as a reservoir for recurrent infection. The purpose of this study was to directly examine the location of Escherichia coli bacteria in the mouse bladder after treatment with mecillinam. Methods: The bladders were studied by use of colony counts, in situ hybridization and electron microscopy. Results: The bacterial counts in the bladder remained ∼103–4 cfu/bladder even after mecillinam treatment had finished, and re-growth in the urine was observed. In the bladder epithelium from treated mice, bacteria cells were occasionally seen, presumably representing intracellularly located bacteria. Conclusions: This is the first in vivo study indicating that during mecillinam treatment E. coli cells can penetrate the mouse bladder epithelium and persist.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dki002