Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider

We have investigated anticholinesterase potential of the methanol extracts from the leaf, wood, flower, twig, and stem bark of the female and male individuals and rhizodermis and fruit from the female tree of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin a...

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Bibliographic Details
Published in:Food and chemical toxicology 2009-08, Vol.47 (8), p.1747-1751
Main Authors: Orhan, I., Şenol, F.S., Kartal, M., Dvorská, M., Žemlička, M., Šmejkal, K., Mokrý, P.
Format: Article
Language:English
Subjects:
acetylcholinesterase
Animals
Anticholinesterase
bark
Benzopyrans - chemistry
Benzopyrans - isolation & purification
Biological and medical sciences
Butyrylcholinesterase - metabolism
chemical constituents of plants
cholinesterase
Cholinesterase Inhibitors - pharmacology
Chromatography, High Pressure Liquid
Electrophorus
enzyme inhibition
Enzyme-Linked Immunosorbent Assay
epidermis (plant)
female flowers
female tree
flowers
fruits (plant anatomy)
Galantamine - pharmacology
iso-osajin
iso-pomiferin
Isoflavone
Isoflavones - chemistry
Isoflavones - isolation & purification
isoflavonoids
LC–DAD–MS
leaves
Maclura - chemistry
Maclura pomifera
Maclura pomifera (Rafin.) Schneider
Mass Spectrometry
Medical sciences
Moraceae
Osage orange
osajin
plant extracts
Plant Extracts - pharmacology
pomiferin
roots
stems
Toxicology
wood
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Summary:We have investigated anticholinesterase potential of the methanol extracts from the leaf, wood, flower, twig, and stem bark of the female and male individuals and rhizodermis and fruit from the female tree of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin as well as their semi-synthetic derivatives; iso-osajin and iso-pomiferin. Anticholinesterase activity was determined by Ellman method using ELISA microplate reader. Osajin and pomiferin had a noticeable inhibition of AChE with IC 50 values of 2.239 and 0.096 mM, respectively, while their iso-derivatives were found to display less inhibition towards AChE. The extracts and compounds did not inhibit BChE. The extracts were analyzed for osajin and pomiferin contents by LC–DAD–MS and only the fruits and female flowers contained osajin (fruit: 8.87%, female flowers: 0.19%, w/w) and pomiferin (fruit: 13.6%, female flowers: 0.36%, w/w).
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2009.04.023