Circulating endothelial cells are elevated in patients with type 2 diabetes mellitus independently of HbA(1)c

Patients with diabetes mellitus are well known to be at high risk for vascular disease. Circulating endothelial cells (CECs) have been reported to be an ex vivo indicator of vascular injury. We investigated the presence of CECs in the peripheral blood of 25 patients with diabetes mellitus and in nin...

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Bibliographic Details
Published in:Diabetologia 2005-02, Vol.48 (2), p.345-350
Main Authors: McClung, J A, Naseer, N, Saleem, M, Rossi, G P, Weiss, M B, Abraham, N G, Kappas, A
Format: Article
Language:English
Subjects:
Adult
Aged
Aging
Antigens, CD - blood
Body Mass Index
CD146 Antigen
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - pathology
Endothelium, Vascular - cytology
Endothelium, Vascular - pathology
Female
Glycated Hemoglobin A - metabolism
Humans
Male
Middle Aged
Neural Cell Adhesion Molecules - blood
Reference Values
Regression Analysis
Umbilical Veins - physiology
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Summary:Patients with diabetes mellitus are well known to be at high risk for vascular disease. Circulating endothelial cells (CECs) have been reported to be an ex vivo indicator of vascular injury. We investigated the presence of CECs in the peripheral blood of 25 patients with diabetes mellitus and in nine non-diabetic control donors. Endothelial cells were isolated from peripheral blood with anti-CD-146-coated immunomagnetic Dynabeads, and were stained with acridine orange dye and counted by fluorescence microscopy. The cells were also stained for von Willebrand factor and Ulex europaeus lectin 1. Patients with diabetes mellitus had an elevated number of CECs (mean 69+/-30 cells/ml, range 35-126) compared with healthy controls (mean 10+/-5 cells/ml, range 3-18) (p
ISSN:0012-186X
DOI:10.1007/s00125-004-1647-5