RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains

RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-bi...

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Published in:Molecules and cells 2005-02, Vol.19 (1), p.39-45
Main Authors: Liu, Lingling (Fudan University, Shanghai, China), Yang, Chenyi (Fudan University, Shanghai, China), Yuan, Jian (Fudan University, Shanghai, China), Chen, Xiujuan (Fudan University, Shanghai, China), Xu, Jianing (Fudan University, Shanghai, China), Wei, Youheng (Fudan University, Shanghai, China), Yang, Jingchun (Fudan University, Shanghai, China), Lin, Gang (Fudan University, Shanghai, China), Yu, Long (Fudan University, Shanghai, China), E-mail: longyu@fudan.edu.cn
Format: Article
Language:English
Subjects:
Animals
Chlorocebus aethiops
Co-immunoprecipitation
Co-localization
COS Cells
Cytoplasm - metabolism
Early Endosome
Endosomes - metabolism
Humans
Intracellular Signaling Peptides and Proteins - chemistry
Intracellular Signaling Peptides and Proteins - metabolism
Neoplasm Proteins - chemistry
Neoplasm Proteins - metabolism
Peroxidases
Peroxiredoxin III
Peroxiredoxins
Phosphotransferases (Alcohol Group Acceptor) - biosynthesis
Phosphotransferases - genetics
PRDX3
Protein Structure, Tertiary
Protein Transport
PROTEINAS
PROTEINE
PROTEINS
Ribosomal Protein S6 Kinases - chemistry
Ribosomal Protein S6 Kinases - metabolism
RPK118
Two-Hybrid System Techniques
Yeast Two-Hybrid
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Summary:RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells.
ISSN:1016-8478
DOI:10.1016/S1016-8478(23)13134-7