Effect of epidermal growth factor in preimplantation development of porcine cloned embryos

In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen...

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Bibliographic Details
Published in:Molecular reproduction and development 2005-05, Vol.71 (1), p.45-51
Main Authors: Lee, Gab Sang, Kim, Hye Soo, Hyun, Sang Hwan, Jeon, Hyun Yong, Nam, Dong Hyun, Jeong, Yeon Woo, Kim, Sue, Kim, Ji hye, Kang, Sung Keun, Lee, Byeong Chun, Hwang, Woo Suk
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastocyst - cytology
Blastocyst - drug effects
Blastocyst - metabolism
Cell Nucleus - genetics
Cell Size
Cells, Cultured
Cloning, Organism
Early stages. Segmentation. Gastrulation. Neurulation
EGF
EGF receptor
Embryology: invertebrates and vertebrates. Teratology
Embryonic Development - drug effects
embryos
Epidermal Growth Factor - genetics
Epidermal Growth Factor - metabolism
Epidermal Growth Factor - pharmacology
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
nuclear transfer
porcine
Receptor, Epidermal Growth Factor - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Swine - embryology
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Summary:In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen‐thawed semen in vitro or reconstructed with fetal fibroblasts by SCNT. In Experiment 1, total RNA was isolated from oocytes, preimplantation SCNT, or in vitro fertilization (IVF) embryos. The expression of EGF and EGFr mRNA was determined using reverse transcription‐polymerase chain reaction (RT‐PCR). In SCNT and IVF embryos, the EGF mRNA was detected in oocytes, 2‐cell, 4‐cell, 8‐cell, morulae, and blastocysts, while EGFr mRNA was detected in oocytes, 2‐cell, morulae, and blastocysts. In Experiment 2, SCNT embryos at 1‐cell stage were cultured in North Carolina State University (NCSU)‐23 medium supplemented with different concentrations of EGF (0.1, 1, or 10 ng/ml). Supplementing with 10 ng/ml EGF improved cleavage rate (82.8% vs. 76.8%, P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20098