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Effect of epidermal growth factor in preimplantation development of porcine cloned embryos
In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen...
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| Published in: | Molecular reproduction and development 2005-05, Vol.71 (1), p.45-51 |
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| cited_by | cdi_FETCH-LOGICAL-c3918-d37431b9648aa9e0df7d56994efa50f70112381ebb934795ceab7796786004673 |
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| cites | cdi_FETCH-LOGICAL-c3918-d37431b9648aa9e0df7d56994efa50f70112381ebb934795ceab7796786004673 |
| container_end_page | 51 |
| container_issue | 1 |
| container_start_page | 45 |
| container_title | Molecular reproduction and development |
| container_volume | 71 |
| creator | Lee, Gab Sang Kim, Hye Soo Hyun, Sang Hwan Jeon, Hyun Yong Nam, Dong Hyun Jeong, Yeon Woo Kim, Sue Kim, Ji hye Kang, Sung Keun Lee, Byeong Chun Hwang, Woo Suk |
| description | In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen‐thawed semen in vitro or reconstructed with fetal fibroblasts by SCNT. In Experiment 1, total RNA was isolated from oocytes, preimplantation SCNT, or in vitro fertilization (IVF) embryos. The expression of EGF and EGFr mRNA was determined using reverse transcription‐polymerase chain reaction (RT‐PCR). In SCNT and IVF embryos, the EGF mRNA was detected in oocytes, 2‐cell, 4‐cell, 8‐cell, morulae, and blastocysts, while EGFr mRNA was detected in oocytes, 2‐cell, morulae, and blastocysts. In Experiment 2, SCNT embryos at 1‐cell stage were cultured in North Carolina State University (NCSU)‐23 medium supplemented with different concentrations of EGF (0.1, 1, or 10 ng/ml). Supplementing with 10 ng/ml EGF improved cleavage rate (82.8% vs. 76.8%, P |
| doi_str_mv | 10.1002/mrd.20098 |
| format | article |
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In vitro matured gilt oocytes were fertilized with frozen‐thawed semen in vitro or reconstructed with fetal fibroblasts by SCNT. In Experiment 1, total RNA was isolated from oocytes, preimplantation SCNT, or in vitro fertilization (IVF) embryos. The expression of EGF and EGFr mRNA was determined using reverse transcription‐polymerase chain reaction (RT‐PCR). In SCNT and IVF embryos, the EGF mRNA was detected in oocytes, 2‐cell, 4‐cell, 8‐cell, morulae, and blastocysts, while EGFr mRNA was detected in oocytes, 2‐cell, morulae, and blastocysts. In Experiment 2, SCNT embryos at 1‐cell stage were cultured in North Carolina State University (NCSU)‐23 medium supplemented with different concentrations of EGF (0.1, 1, or 10 ng/ml). Supplementing with 10 ng/ml EGF improved cleavage rate (82.8% vs. 76.8%, P < 0.05), but not the rate of blastocyst formation compared to the control. At all concentrations, EGF increased (P < 0.05) the total cell number in blastocysts (range 50.5–53.7 vs. 43.9). In Experiment 3, EGF (10 ng/ml) was added to NCSU‐23 medium at the morula stage. The EGF did not affect blastocyst formation, total cell number in blastocysts or the ratio of inner cell mass (ICM) to total cell number. In conclusion, we demonstrated that EGF and EGFr mRNA are expressed in porcine IVF and SCNT preimplantation embryos, and that EGF increased the quality of blastocysts by increasing total cell numbers in porcine SCNT embryos. Mol. Reprod. Dev. 71: 45–51, 2005. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.20098</identifier><identifier>PMID: 15736135</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - metabolism ; Cell Nucleus - genetics ; Cell Size ; Cells, Cultured ; Cloning, Organism ; Early stages. Segmentation. Gastrulation. Neurulation ; EGF ; EGF receptor ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic Development - drug effects ; embryos ; Epidermal Growth Factor - genetics ; Epidermal Growth Factor - metabolism ; Epidermal Growth Factor - pharmacology ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; nuclear transfer ; porcine ; Receptor, Epidermal Growth Factor - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Swine - embryology</subject><ispartof>Molecular reproduction and development, 2005-05, Vol.71 (1), p.45-51</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright (c) 2005 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3918-d37431b9648aa9e0df7d56994efa50f70112381ebb934795ceab7796786004673</citedby><cites>FETCH-LOGICAL-c3918-d37431b9648aa9e0df7d56994efa50f70112381ebb934795ceab7796786004673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16654317$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15736135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Gab Sang</creatorcontrib><creatorcontrib>Kim, Hye Soo</creatorcontrib><creatorcontrib>Hyun, Sang Hwan</creatorcontrib><creatorcontrib>Jeon, Hyun Yong</creatorcontrib><creatorcontrib>Nam, Dong Hyun</creatorcontrib><creatorcontrib>Jeong, Yeon Woo</creatorcontrib><creatorcontrib>Kim, Sue</creatorcontrib><creatorcontrib>Kim, Ji hye</creatorcontrib><creatorcontrib>Kang, Sung Keun</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Hwang, Woo Suk</creatorcontrib><title>Effect of epidermal growth factor in preimplantation development of porcine cloned embryos</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen‐thawed semen in vitro or reconstructed with fetal fibroblasts by SCNT. In Experiment 1, total RNA was isolated from oocytes, preimplantation SCNT, or in vitro fertilization (IVF) embryos. The expression of EGF and EGFr mRNA was determined using reverse transcription‐polymerase chain reaction (RT‐PCR). In SCNT and IVF embryos, the EGF mRNA was detected in oocytes, 2‐cell, 4‐cell, 8‐cell, morulae, and blastocysts, while EGFr mRNA was detected in oocytes, 2‐cell, morulae, and blastocysts. In Experiment 2, SCNT embryos at 1‐cell stage were cultured in North Carolina State University (NCSU)‐23 medium supplemented with different concentrations of EGF (0.1, 1, or 10 ng/ml). Supplementing with 10 ng/ml EGF improved cleavage rate (82.8% vs. 76.8%, P < 0.05), but not the rate of blastocyst formation compared to the control. At all concentrations, EGF increased (P < 0.05) the total cell number in blastocysts (range 50.5–53.7 vs. 43.9). In Experiment 3, EGF (10 ng/ml) was added to NCSU‐23 medium at the morula stage. The EGF did not affect blastocyst formation, total cell number in blastocysts or the ratio of inner cell mass (ICM) to total cell number. In conclusion, we demonstrated that EGF and EGFr mRNA are expressed in porcine IVF and SCNT preimplantation embryos, and that EGF increased the quality of blastocysts by increasing total cell numbers in porcine SCNT embryos. Mol. Reprod. Dev. 71: 45–51, 2005. © 2005 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - metabolism</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Size</subject><subject>Cells, Cultured</subject><subject>Cloning, Organism</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>EGF</subject><subject>EGF receptor</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic Development - drug effects</subject><subject>embryos</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>nuclear transfer</subject><subject>porcine</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Swine - embryology</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNp1kE9P3DAQxa2qqMC2h34B5EuROATsOLbjY_mPgFaqqFr1YjnOmLpN4mBngf32eNmlnHqaOfzevHkPoY-U7FNCyoM-tvslIap-g7ZoHkUpFX-73CtSVLz8uYm2U_pDMqJq8g5tUi6ZoIxvoV8nzoGdcHAYRt9C7E2Hb2N4mH5jZ-wUIvYDHiP4fuzMMJnJhwG3cA9dGHsYnpVjiNYPgG0XBmgx9E1chPQebTjTJfiwnjP0_fTk5ui8uPp6dnH0-aqwTNG6aJmsGG2UqGpjFJDWyZYLpSpwhhMnCaUlqyk0jWJVzmXBNFIqIWtBSCUkm6Hd1d0xhrs5pEn3Plno8rsQ5kkLWdWKZ5MZ2luBNoaUIjg9Rt-buNCU6GWROhepn4vM7M766LzpoX0l181l4NMaMMmazkUzWJ9eOSF4jrX87mDFPfgOFv931Nffjl-si5XCpwke_ylM_JujMMn1jy9n-vCaHpanlzeasCfP0Zj1</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Lee, Gab Sang</creator><creator>Kim, Hye Soo</creator><creator>Hyun, Sang Hwan</creator><creator>Jeon, Hyun Yong</creator><creator>Nam, Dong Hyun</creator><creator>Jeong, Yeon Woo</creator><creator>Kim, Sue</creator><creator>Kim, Ji hye</creator><creator>Kang, Sung Keun</creator><creator>Lee, Byeong Chun</creator><creator>Hwang, Woo Suk</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Effect of epidermal growth factor in preimplantation development of porcine cloned embryos</title><author>Lee, Gab Sang ; Kim, Hye Soo ; Hyun, Sang Hwan ; Jeon, Hyun Yong ; Nam, Dong Hyun ; Jeong, Yeon Woo ; Kim, Sue ; Kim, Ji hye ; Kang, Sung Keun ; Lee, Byeong Chun ; Hwang, Woo Suk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-d37431b9648aa9e0df7d56994efa50f70112381ebb934795ceab7796786004673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - metabolism</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Size</topic><topic>Cells, Cultured</topic><topic>Cloning, Organism</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>EGF</topic><topic>EGF receptor</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic Development - drug effects</topic><topic>embryos</topic><topic>Epidermal Growth Factor - genetics</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>nuclear transfer</topic><topic>porcine</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Swine - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Gab Sang</creatorcontrib><creatorcontrib>Kim, Hye Soo</creatorcontrib><creatorcontrib>Hyun, Sang Hwan</creatorcontrib><creatorcontrib>Jeon, Hyun Yong</creatorcontrib><creatorcontrib>Nam, Dong Hyun</creatorcontrib><creatorcontrib>Jeong, Yeon Woo</creatorcontrib><creatorcontrib>Kim, Sue</creatorcontrib><creatorcontrib>Kim, Ji hye</creatorcontrib><creatorcontrib>Kang, Sung Keun</creatorcontrib><creatorcontrib>Lee, Byeong Chun</creatorcontrib><creatorcontrib>Hwang, Woo Suk</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Gab Sang</au><au>Kim, Hye Soo</au><au>Hyun, Sang Hwan</au><au>Jeon, Hyun Yong</au><au>Nam, Dong Hyun</au><au>Jeong, Yeon Woo</au><au>Kim, Sue</au><au>Kim, Ji hye</au><au>Kang, Sung Keun</au><au>Lee, Byeong Chun</au><au>Hwang, Woo Suk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of epidermal growth factor in preimplantation development of porcine cloned embryos</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2005-05</date><risdate>2005</risdate><volume>71</volume><issue>1</issue><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, and the effect of exogenous EGF supplementation on preimplantation development of porcine somatic cell nuclear transfer (SCNT) embryos. In vitro matured gilt oocytes were fertilized with frozen‐thawed semen in vitro or reconstructed with fetal fibroblasts by SCNT. In Experiment 1, total RNA was isolated from oocytes, preimplantation SCNT, or in vitro fertilization (IVF) embryos. The expression of EGF and EGFr mRNA was determined using reverse transcription‐polymerase chain reaction (RT‐PCR). In SCNT and IVF embryos, the EGF mRNA was detected in oocytes, 2‐cell, 4‐cell, 8‐cell, morulae, and blastocysts, while EGFr mRNA was detected in oocytes, 2‐cell, morulae, and blastocysts. In Experiment 2, SCNT embryos at 1‐cell stage were cultured in North Carolina State University (NCSU)‐23 medium supplemented with different concentrations of EGF (0.1, 1, or 10 ng/ml). Supplementing with 10 ng/ml EGF improved cleavage rate (82.8% vs. 76.8%, P < 0.05), but not the rate of blastocyst formation compared to the control. At all concentrations, EGF increased (P < 0.05) the total cell number in blastocysts (range 50.5–53.7 vs. 43.9). In Experiment 3, EGF (10 ng/ml) was added to NCSU‐23 medium at the morula stage. The EGF did not affect blastocyst formation, total cell number in blastocysts or the ratio of inner cell mass (ICM) to total cell number. In conclusion, we demonstrated that EGF and EGFr mRNA are expressed in porcine IVF and SCNT preimplantation embryos, and that EGF increased the quality of blastocysts by increasing total cell numbers in porcine SCNT embryos. Mol. Reprod. Dev. 71: 45–51, 2005. © 2005 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15736135</pmid><doi>10.1002/mrd.20098</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 2005-05, Vol.71 (1), p.45-51 |
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| subjects | Animals Biological and medical sciences Blastocyst - cytology Blastocyst - drug effects Blastocyst - metabolism Cell Nucleus - genetics Cell Size Cells, Cultured Cloning, Organism Early stages. Segmentation. Gastrulation. Neurulation EGF EGF receptor Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects embryos Epidermal Growth Factor - genetics Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology Fertilization in Vitro Fundamental and applied biological sciences. Psychology nuclear transfer porcine Receptor, Epidermal Growth Factor - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Swine - embryology |
| title | Effect of epidermal growth factor in preimplantation development of porcine cloned embryos |
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