RNase J is involved in the 5′-end maturation of 16S rRNA and 23S rRNA in Sinorhizobium meliloti

• Published in: FEBS letters 2009-07, Vol.583 (14), p.2339-2342
• Main Authors: Madhugiri, Ramakanth, Evguenieva-Hackenberg, Elena
• Format: Article
• Language: English
• Subjects: 16S rRNA; 23S rRNA; 5′-End; Bacillus subtilis; Base Sequence; Endoribonucleases - genetics; Endoribonucleases - metabolism; Escherichia coli; Genetic Complementation Test; Isoenzymes - genetics; Isoenzymes - metabolism; Molecular Sequence Data; Nucleic Acid Conformation; nucleotide; rDNA; Ribonuclease; Ribonucleases - genetics; Ribonucleases - metabolism; ribosomal RNA; ribosomal RNA operon; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - metabolism; RNA, Ribosomal, 23S - chemistry; RNA, Ribosomal, 23S - genetics; RNA, Ribosomal, 23S - metabolism; RNA, Ribosomal, 5S - chemistry; RNA, Ribosomal, 5S - genetics; RNA, Ribosomal, 5S - metabolism; RNase; RNase J; rrn; rRNA; rRNA gene; Sinorhizobium meliloti; Sinorhizobium meliloti - genetics; Small RNA
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2009.06.026

Sinorhizobium meliloti harbours genes encoding orthologs of ribonuclease (RNase) E and RNase J, the principle endoribonucleases in Escherichia coli and Bacillus subtilis, respectively. To analyse the role of RNase J in S. meliloti, RNA from a mutant with miniTn5-insertion in the RNase J-encoding gene was compared to the wild-type and a difference in the length of the 5.8S-like ribosomal RNA (rRNA) was observed. Complementation of the mutant, Northern blotting and primer extension revealed that RNase J is necessary for the 5′-end maturation of 16S rRNA and of the two 23S rRNA fragments, but not of 5S rRNA.