Tetra primer ARMS-PCR for identification of SNP in β-tubulin of Botrytis cinerea, responsible of resistance to benzimidazole

A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in β-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene,...

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Bibliographic Details
Published in:Journal of microbiological methods 2009-08, Vol.78 (2), p.245-246
Main Authors: Muñoz, Claudio, Gómez Talquenca, Sebastián, Volpe, Melisa Lanza
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Benzimidazole
Benzimidazoles - pharmacology
Biological and medical sciences
Botrytis - drug effects
Botrytis - genetics
Botrytis cinerea
DNA Primers - genetics
DNA, Fungal - genetics
Drug Resistance, Fungal
Fundamental and applied biological sciences. Psychology
Microbiology
Mycological methods and techniques used in mycology
Mycology
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Resistance
SNP
Tetra primer ARMS-PCR
Tubulin - genetics
β-tubulin
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Summary:A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in β-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene, which was readily identified by the technique and in the six cases correspond to the resistant strain.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2009.06.007