Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model

To understand causes of developmental abnormalities of the pancreas, it is essential to understand its normal embryonic development. Current understanding of the development of pancreatic exocrine tissue is that it develops solely from embryonic epithelium, while the role of the surrounding mesenchy...

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Bibliographic Details
Published in:Pediatric surgery international 2005-03, Vol.21 (3), p.138-142
Main Authors: Teague, Warwick J, Jayanthi, Naga V G, Lear, Pamela V, Johnson, Paul R V
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Chick Embryo
Chimera
Epithelium - embryology
Epithelium - ultrastructure
Immunohistochemistry
Islets of Langerhans - embryology
Islets of Langerhans - ultrastructure
Mesoderm - ultrastructure
Microscopy, Fluorescence
Models, Biological
Pancreas, Exocrine - embryology
Pancreas, Exocrine - ultrastructure
Quail
Tissue Culture Techniques
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Summary:To understand causes of developmental abnormalities of the pancreas, it is essential to understand its normal embryonic development. Current understanding of the development of pancreatic exocrine tissue is that it develops solely from embryonic epithelium, while the role of the surrounding mesenchyme is to signal to this epithelium and form connective tissue. Recent work in our laboratory has shown that pancreatic bud mesenchyme can contribute cells to islets during embryonic development. However, no published studies have investigated in detail whether mesenchyme contributes cells to the exocrine structures of the pancreas. The aim of this study was to investigate whether cells from foregut mesenchyme can contribute to pancreatic acini during embryonic development. Chick-quail chimera recombinant organs were constructed using pancreatic epithelium and mesenchyme from either the pancreas (n=12) or stomach (n=25). These were cultured for 7 days in 3-D collagen gels. The resulting specimens were analysed using morphological criteria and fluorescent immunocytochemistry against pancreatic amylase, insulin, and the quail-specific nucleolar antigen QCPN. Two independent observers determined the origins of acini as either solely epithelial, solely mesenchymal, or of mixed origin. Results are expressed as percentages of total acini identified in each group. Statistical analysis was performed using chi(2) tests (P
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-004-1309-1