Bacterial translocation in acute rejection after small bowel transplantation in rats

Acute rejection after small bowel transplantation (SBTx) may facilitate bacterial translocation (BT) and subsequent changes in the liver, spleen, and lungs. This study investigated whether BT occurs after acute rejection and whether this is followed by changes in the structure of the intestine and t...

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Bibliographic Details
Published in:Pediatric surgery international 2005-03, Vol.21 (3), p.208-211
Main Authors: Zou, Y, Hernandez, F, Burgos, E, Martinez, L, Gonzalez-Reyes, S, Fernandez-Dumont, V, Lopez, G, Romero, M, Lopez-Santamaria, M, Tovar, J A
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Bacterial Translocation
beta-Galactosidase - genetics
beta-Galactosidase - metabolism
Biomarkers - analysis
Colony Count, Microbial
Culture Media
Disease Models, Animal
DNA, Bacterial - genetics
Escherichia coli - enzymology
Escherichia coli - isolation & purification
Escherichia coli - physiology
Graft Rejection - metabolism
Graft Rejection - microbiology
Graft Rejection - pathology
Intestine, Small - chemistry
Intestine, Small - microbiology
Intestine, Small - pathology
Intestine, Small - transplantation
Liver - chemistry
Lung - chemistry
Lymph Nodes - chemistry
Lymph Nodes - pathology
Mesentery
Proteins - analysis
Rats
Rats, Inbred BN
Rats, Wistar
Spectrometry, Fluorescence
Spleen - chemistry
Tissue Culture Techniques
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Summary:Acute rejection after small bowel transplantation (SBTx) may facilitate bacterial translocation (BT) and subsequent changes in the liver, spleen, and lungs. This study investigated whether BT occurs after acute rejection and whether this is followed by changes in the structure of the intestine and the phagocytic organs interposed between the gut and the general circulation. Orthotopic SBTx was performed in allogeneic (ALLO) rat-strain combinations (BN-Wistar, n=5). For comparison we used syngeneic SBTx (SYN) (BN-BN, n=6) controls. Animals were sacrificed on postoperative day 7. Mesenteric lymph nodes and portal and caval blood were cultured for aerobes and anaerobes. Escherichia coli beta-galactosidase DNA was assessed by polymerase chain reaction in the blood samples. Intestine, liver, spleen, and lung protein and DNA contents were measured. Histologic changes were graded according to standard criteria of acute rejection. For comparisons we used chi(2) and nonparametric Mann-Whitney test with a threshold of significance of p
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-004-1321-5