Densitometric high performance thin-layer chromatography identification and quantitative analysis of psychotropic drugs

A thin-layer chromatography (TLC)-densitometry method has been developed to identify and quantify haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in selected psychotropic drugs. Separation was performed on precoated s...

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Bibliographic Details
Published in:Journal of AOAC International 2005, Vol.88 (1), p.70-79
Main Authors: MASLANKA, Anna, KRZEK, Jan
Format: Article
Language:English
Subjects:
Amitriptyline - analysis
Analysis
Antipsychotic Agents - analysis
Biological and medical sciences
Chemistry Techniques, Analytical - methods
Chlorpromazine - analysis
Chromatography
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
Clonazepam - analysis
Densitometry
Densitometry - methods
Diazepam - analysis
Doxepin - analysis
Drugs
Fluphenazine - analysis
General pharmacology
Haloperidol - analysis
Identification and classification
Measurement
Medical sciences
Methods
Pharmaceutical Preparations - analysis
Pharmacology. Drug treatments
Product/Service Evaluations
Promazine - analysis
Psychotropic drugs
Psychotropic Drugs - analysis
Regression Analysis
Sulpiride - analysis
Thin layer chromatography
Trifluoperazine - analysis
Ultraviolet Rays
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Summary:A thin-layer chromatography (TLC)-densitometry method has been developed to identify and quantify haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in selected psychotropic drugs. Separation was performed on precoated silica gel 60 F254 TLC plates. Chromatograms were developed in various mobile phases, and 8 of 30 tested phases were selected based on spot location and developing time. The identification and quantification were carried out based on ultraviolet densitometric measurements at chosen wavelengths. In addition to retention coefficients, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Under established experimental conditions, high sensitivity of the method was achieved. The limit of detection ranged from 0.009 to 0.260 microg, depending on the wavelength selected for measuring. A satisfactory recovery, ranging from 92.99 to 104.70%, was achieved for individual constituents.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/88.1.70