miR-184 Has Multiple Roles in Drosophila Female Germline Development

Posttranscriptional regulation plays a crucial role in germline and early embryonic development, but the underlying mechanisms are only partially understood. Here we report the genetic and molecular analysis of the maternally and zygotically expressed microRNA miR-184 in Drosophila. Loss of miR-184...

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Bibliographic Details
Published in:Developmental cell 2009-07, Vol.17 (1), p.123-133
Main Authors: Iovino, Nicola, Pane, Attilio, Gaul, Ulrike
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Animals
Base Sequence
Biological and medical sciences
Body Patterning
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
DEVBIO
Drosophila melanogaster - classification
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Germ Cells - cytology
Germ Cells - physiology
Humans
MicroRNAs - genetics
MicroRNAs - metabolism
Molecular and cellular biology
Molecular Sequence Data
Oocytes - cytology
Oocytes - physiology
Oogenesis - physiology
RNA
Stem Cells - physiology
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Summary:Posttranscriptional regulation plays a crucial role in germline and early embryonic development, but the underlying mechanisms are only partially understood. Here we report the genetic and molecular analysis of the maternally and zygotically expressed microRNA miR-184 in Drosophila. Loss of miR-184 leads to multiple severe defects during oogenesis and early embryogenesis, culminating in the complete loss of egg production. Using both in vitro and in vivo assays, we characterize the relevant miR-184 targets and target sites for three of the observed phenotypes. miR-184 controls germline stem cell differentiation by tuning the DPP receptor Saxophone, dorsoventral patterning of the egg shell by regulating the gurken transport factor K10, and anteroposterior patterning of the blastoderm by tuning the transcriptional repressor Tramtrack69. Our study highlights the importance of microRNA-mediated regulation in the major developmental transitions of the female germline, and provides insights into several aspects of microRNA function.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2009.06.008