Analysis of macrophage migration inhibitory factor (MIF) in patients with idiopathic pulmonary fibrosis

Abstract By proteomic approach we previously characterised bronchoalveolar lavage (BAL) protein profiles of patients with idiopathic pulmonary fibrosis (IPF), sarcoidosis and systemic sclerosis. Among differently expressed proteins we identified macrophage migration inhibitory factor (MIF), a multi-...

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Bibliographic Details
Published in:Respiratory physiology & neurobiology 2009-07, Vol.167 (3), p.261-267
Main Authors: Bargagli, E, Olivieri, C, Nikiforakis, N, Cintorino, M, Magi, B, Perari, M.G, Vagaggini, C, Spina, D, Prasse, A, Rottoli, P
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Blood Gas Analysis
Bronchoalveolar lavage
Bronchoalveolar Lavage Fluid - cytology
Electrophoresis, Gel, Two-Dimensional
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Humans
Idiopathic pulmonary fibrosis
Immunohistochemistry
Interstitial lung diseases
Lung tissue
Macrophage migration inhibitory factor
Macrophage Migration-Inhibitory Factors - blood
Male
Medical Education
Middle Aged
Pulmonary Fibrosis - blood
Pulmonary/Respiratory
Respiratory Function Tests
Smoking - adverse effects
Vertebrates: respiratory system
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Summary:Abstract By proteomic approach we previously characterised bronchoalveolar lavage (BAL) protein profiles of patients with idiopathic pulmonary fibrosis (IPF), sarcoidosis and systemic sclerosis. Among differently expressed proteins we identified macrophage migration inhibitory factor (MIF), a multi-function pleiotropic cytokine. This study was performed to validate our findings by a further proteomic approach and ELISA in a larger population of patients and controls. MIF expression in lung tissue was also evaluated by immunohistochemistry. MIF was identified in all 2-DE gels of IPF patients and it was significantly increased compared to controls ( p < 0.05). This result was confirmed by ELISA: MIF concentrations were significantly higher in IPF patients than controls ( p < 0.001) and were directly correlated with neutrophil percentages ( p = 0.0095). Immunohistochemical analysis revealed enhanced expression in bronchiolar epithelium, alveolar epithelium, and fibroblastic foci. In conclusion, MIF is a pleiotropic cytokine that could be involved in the pathogenesis of IPF, being particularly abundant in BAL of these patients and mainly expressed in the areas of active fibrosis.
ISSN:1569-9048
1878-1519
DOI:10.1016/j.resp.2009.05.004